Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Leishmania infantum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N,N,N-trimethyl-N-(5,5-diphenylpent-4-en-1-yl)ammonium iodide | - |
Leishmania infantum | |
N,N,N-trimethyl-N-(6,6-diphenylhex-5-en-1-yl)ammonium iodide | - |
Leishmania infantum | |
N-iodomethyl-N,N-dimethyl-N-(4,4-diphenylbut-3-en-1-yl)ammonium iodide | - |
Leishmania infantum | |
N-iodomethyl-N,N-dimethyl-N-(5,5-diphenylpent-4-en-1-yl)ammonium iodide | - |
Leishmania infantum | |
N-iodomethyl-N,N-dimethyl-N-(6,6-diphenylhex-5-en-1-yl)ammonium iodide | - |
Leishmania infantum | |
N-iodomethylcholine iodide | - |
Leishmania infantum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.089 | - |
choline | at pH 9.0 and 37°C | Leishmania infantum | |
75 | - |
ethanolamine | at pH 9.0 and 37°C | Leishmania infantum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 11 mM used in assay conditions | Leishmania infantum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania infantum | A4IB50 | - |
- |
Leishmania infantum JPCM5 | A4IB50 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography | Leishmania infantum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
promastigote | - |
Leishmania infantum | - |
Storage Stability | Organism |
---|---|
-20°C, 50 mM Tris and 50 mM glycine pH 9.0, 24 h, 30-50% loss of activity | Leishmania infantum |
-80°C, 50 mM Tris and 50 mM glycine pH 9.0, with or without freeze drying, 24 h, about 50% loss of activity | Leishmania infantum |
4°C, 50 mM Tris and 50 mM glycine pH 9.0, 24 h, no loss of activity | Leishmania infantum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + choline | the catalytic efficiency (Vmax/Km) using choline as substrate is 2000fold greater than the efficiency using ethanolamine as substrate | Leishmania infantum | ADP + O-phosphocholine | - |
? | |
ATP + choline | the catalytic efficiency (Vmax/Km) using choline as substrate is 2000fold greater than the efficiency using ethanolamine as substrate | Leishmania infantum JPCM5 | ADP + O-phosphocholine | - |
? | |
ATP + ethanolamine | - |
Leishmania infantum | ADP + O-phosphoethanolamine | - |
? | |
ATP + ethanolamine | - |
Leishmania infantum JPCM5 | ADP + O-phosphoethanolamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 79000, SDS-PAGE | Leishmania infantum |
Synonyms | Comment | Organism |
---|---|---|
LinfC/EK35 | the enzyme has choline kinase and ethanolamine kinase activities | Leishmania infantum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Leishmania infantum |