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Literature summary for 2.7.1.32 extracted from

  • Pulido, S.A.; Nguyen, V.H.; Alzate, J.F.; Cedeno, D.L.; Makurath, M.A.; Rios-Vasquez, A.; Duque-Benitez, S.M.; Jones, M.A.; Robledo, S.M.; Friesen, J.A.
    Insights into the phosphatidylcholine and phosphatidylethanolamine biosynthetic pathways in Leishmania parasites and characterization of a choline kinase from Leishmania infantum (2017), Comp. Biochem. Physiol. B, 213, 45-54 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Leishmania infantum

Inhibitors

Inhibitors Comment Organism Structure
N,N,N-trimethyl-N-(5,5-diphenylpent-4-en-1-yl)ammonium iodide
-
Leishmania infantum
N,N,N-trimethyl-N-(6,6-diphenylhex-5-en-1-yl)ammonium iodide
-
Leishmania infantum
N-iodomethyl-N,N-dimethyl-N-(4,4-diphenylbut-3-en-1-yl)ammonium iodide
-
Leishmania infantum
N-iodomethyl-N,N-dimethyl-N-(5,5-diphenylpent-4-en-1-yl)ammonium iodide
-
Leishmania infantum
N-iodomethyl-N,N-dimethyl-N-(6,6-diphenylhex-5-en-1-yl)ammonium iodide
-
Leishmania infantum
N-iodomethylcholine iodide
-
Leishmania infantum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.089
-
choline at pH 9.0 and 37°C Leishmania infantum
75
-
ethanolamine at pH 9.0 and 37°C Leishmania infantum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 11 mM used in assay conditions Leishmania infantum

Organism

Organism UniProt Comment Textmining
Leishmania infantum A4IB50
-
-
Leishmania infantum JPCM5 A4IB50
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography Leishmania infantum

Source Tissue

Source Tissue Comment Organism Textmining
promastigote
-
Leishmania infantum
-

Storage Stability

Storage Stability Organism
-20°C, 50 mM Tris and 50 mM glycine pH 9.0, 24 h, 30-50% loss of activity Leishmania infantum
-80°C, 50 mM Tris and 50 mM glycine pH 9.0, with or without freeze drying, 24 h, about 50% loss of activity Leishmania infantum
4°C, 50 mM Tris and 50 mM glycine pH 9.0, 24 h, no loss of activity Leishmania infantum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + choline the catalytic efficiency (Vmax/Km) using choline as substrate is 2000fold greater than the efficiency using ethanolamine as substrate Leishmania infantum ADP + O-phosphocholine
-
?
ATP + choline the catalytic efficiency (Vmax/Km) using choline as substrate is 2000fold greater than the efficiency using ethanolamine as substrate Leishmania infantum JPCM5 ADP + O-phosphocholine
-
?
ATP + ethanolamine
-
Leishmania infantum ADP + O-phosphoethanolamine
-
?
ATP + ethanolamine
-
Leishmania infantum JPCM5 ADP + O-phosphoethanolamine
-
?

Subunits

Subunits Comment Organism
? x * 79000, SDS-PAGE Leishmania infantum

Synonyms

Synonyms Comment Organism
LinfC/EK35 the enzyme has choline kinase and ethanolamine kinase activities Leishmania infantum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Leishmania infantum