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Literature summary for 2.7.1.32 extracted from

  • Wang, L.; Jiang, Y.L.; Zhang, J.R.; Zhou, C.Z.; Chen, Y.
    Structural and enzymatic characterization of the choline kinase LicA from Streptococcus pneumoniae (2015), PLoS ONE, 10, e0120467.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.32

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptococcus pneumoniae

Crystallization (Commentary)

Crystallization (Comment) Organism
native and selenomethionine-labelled enzyme, hanging drop vapor diffusion method, using 0.1M HEPES, pH 7.5, 1.2 M sodium citrate and 4% (v/v) glycerol Streptococcus pneumoniae

Protein Variants

Protein Variants Comment Organism
D167A the mutant shows about 14% activity compared to the wild type enzyme Streptococcus pneumoniae
T29A the mutant shows about 10% activity compared to the wild type enzyme Streptococcus pneumoniae
T29S the mutant shows slightly increased activity compared to the wild type enzyme Streptococcus pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
EDTA about 10% residual activity at 5 mM Streptococcus pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 choline at pH 8.0 and 37°C Streptococcus pneumoniae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Streptococcus pneumoniae 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + choline Streptococcus pneumoniae
-
 ADP + phosphocholine
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae Q8DPI4
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 200 gel filtration Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + choline
-
 Streptococcus pneumoniae ADP + phosphocholine
-
 ?

Synonyms

Synonyms Comment Organism
LicA
-
 Streptococcus pneumoniae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1
-
 choline at pH 8.0 and 37°C Streptococcus pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Streptococcus pneumoniae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
33.3
-
 choline at pH 8.0 and 37°C Streptococcus pneumoniae