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EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Reference
3.2.1.35
-999
-
hyaluronan
kinetic measurement on hyaluronan substrates with sizes ranging from 90 to 752 kDa. Km-values: 0.87-0.91 mg/ml. A simple and sensitive method is developed for kinetic measurement of recombinant human hyaluronidase PH20 (rHuPH20) on hyaluronan substrates with sizes ranging from 90 to 752 kDa. The method is based on 2-aminobenzamide labeling of hydrolyzed hyaluronan products combined with separation by size exclusion-ultra performance liquid chromatography coupled with fluorescence detection
749559
3.2.1.35
-999
-
hyaluronic acid
Km: 0.00848 mg/ml, at 37°C, pH 8.0
751278
3.2.1.35
-999
-
hyaluronoic acid
0.48 mg/ml
171394
3.2.1.35
-999
-
hyaluronoic acid
0.71 mg/ml
171402
3.2.1.35
-999
-
hyaluronoic acid
value in mg/ml
171394
,
171402
3.2.1.35
-999
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more
kinetics
649321
3.2.1.35
-999
-
more
Km (hyaluronan) (microgram/ml): 4.91, Vmax (hyaluronan): 2.02 U/min
701325
3.2.1.35
-999
-
more
Km strongly decreases with increasing HA chain sizes
699963
3.2.1.35
0.00091
-
hyaluronic acid
in 5 mM ammonium acetate, at 37°C and pH 5.0
681742
3.2.1.35
0.038
-
hyaluronan
Hyal1 wild-type
693261
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