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Literature summary for 3.2.1.35 extracted from

  • Fang, S.; Hays Putnam, A.M.; LaBarre, M.J.
    Kinetic investigation of recombinant human hyaluronidase PH20 on hyaluronic acid (2015), Anal. Biochem., 480, 74-81 .
    View publication on PubMed
Search for more literature for 3.2.1.35

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 hyaluronan kinetic measurement on hyaluronan substrates with sizes ranging from 90 to 752 kDa. Km-values: 0.87-0.91 mg/ml. A simple and sensitive method is developed for kinetic measurement of recombinant human hyaluronidase PH20 (rHuPH20) on hyaluronan substrates with sizes ranging from 90 to 752 kDa. The method is based on 2-aminobenzamide labeling of hydrolyzed hyaluronan products combined with separation by size exclusion-ultra performance liquid chromatography coupled with fluorescence detection Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P38567
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hyaluronan + H2O
-
 Homo sapiens ?
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 ?

Synonyms

Synonyms Comment Organism
hyaluronidase PH20
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 hyaluronan kinetic measurement on hyaluronan substrates with sizes ranging from 90 to 752 kDa. kcat: 40.5-42.4/s. A simple and sensitive method is developed for kinetic measurement of recombinant human hyaluronidase PH20 (rHuPH20) on hyaluronan substrates with sizes ranging from 90 to 752 kDa. The method is based on 2-aminobenzamide labeling of hydrolyzed hyaluronan products combined with separation by size exclusion-ultra performance liquid chromatography coupled with fluorescence detection Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5.5
-
 Homo sapiens