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Literature summary for 3.2.1.35 extracted from

  • Krishnapillai, A.M.; Taylor, K.D.A.; Morris, A.E.J.; Quantick, P.C.
    Characterisation of Norway lobster (Nephrops norvegicus) hyaluronidase and comparison with sheep and bovine testicular hyaluronidase (1999), Food Chem., 65, 515-521.
No PubMed abstract available
Search for more literature for 3.2.1.35

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin enhanced activity of 22% at 0.26 mg/ml Nephrops norvegicus
EDTA up to 24% enhanced activity Nephrops norvegicus
NaCl 0.12 M required for optimum activity Nephrops norvegicus

General Stability

General Stability Organism
enzyme activity lost by freezing, addition of bovine serum albumin or 1% dimethylsulfoxide, v/v, prevents inactivation Nephrops norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 82% inhibition at 0.8 mM Nephrops norvegicus
chondroitin O-sulfate complete inhibition from 0.012-1.2 mg/ml Nephrops norvegicus
Cu2+ complete inhibition at 0.8 mM Nephrops norvegicus
cysteine complete inhibition from 0.012-1.2 mg/ml Nephrops norvegicus
Fe2+ complete inhibition at 0.8 mM Nephrops norvegicus
Fe3+ complete inhibition at 0.8 mM Nephrops norvegicus
heparin 58.4% inhibition at 1.2 mg/ml Nephrops norvegicus
Hg2+ complete inhibition at 0.8 mM Nephrops norvegicus
serum protein from human, 33.7% inhibition at 1.2 mg/ml Nephrops norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 hyaluronoic acid value in mg/ml Nephrops norvegicus
additional information
-
 hyaluronoic acid 0.48 mg/ml Nephrops norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 18% enhanced activity at 0.8 mM Nephrops norvegicus
NO3- 18% enhanced activity at 0.8 mM Nephrops norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
320000
-
 gel filtration, native PAGE Nephrops norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hyaluronic acid + H2O Nephrops norvegicus endo-mucopolysaccharidase hydrolysing acid mucopolysaccharides from connective tissue and chondroitin-sulfate in cartilage ?
-
 ?

Organism

Organism UniProt Comment Textmining
Nephrops norvegicus
-
 Norway lobster
-

Purification (Commentary)

Purification (Comment) Organism
partial Nephrops norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
hepatopancreas
-
 Nephrops norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O 12 h incubation Nephrops norvegicus ?
-
 ?
hyaluronic acid + H2O endo-mucopolysaccharidase hydrolysing acid mucopolysaccharides from connective tissue and chondroitin-sulfate in cartilage Nephrops norvegicus ?
-
 ?
hyaluronic acid + H2O
-
 Nephrops norvegicus hyaluronic acid oligomers
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 sharp decrease of activity above Nephrops norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4
-
 in 50 mM sodium-acetate buffer, optimum pH depends on buffer used Nephrops norvegicus