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EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Reference
3.4.21.62
0.85
-
acetyl-L-Phe
30°C, pH 8.0
668316
3.4.21.62
0.22
-
acetyl-L-Phe ethyl ester
30°C, pH 8.0
668316
3.4.21.62
4
-
acetyl-L-Tyr
30°C, pH 8.0
668316
3.4.21.62
0.37
-
acetyl-L-Tyr ethyl ester
30°C, pH 8.0
668316
3.4.21.62
7
-
benzoyl-Arg ethyl ester
subtilisin Novo, subtilisin Carlsberg
29425
3.4.21.62
10
-
benzoyl-Arg ethyl ester
subtilisn BPN'
29425
3.4.21.62
2.1
-
benzoyl-L-Arg ethyl ester
30°C, pH 8.0
668316
3.4.21.62
8.3
-
benzyloxycarbonyl-Gly-4-nitroanilide
pH 7.5, 20°C, in presence and absence of DTT
649692
3.4.21.62
0.0054
-
benzyloxycarbonyl-L-Ala-L-Ala-L-Leu-4-nitroanilide
-
667756
3.4.21.62
0.059
-
casein
pH 10, 45°C, Vmax: 0.336 mg/min
717205
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