EC Number   |
KM Value [mM]  |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
   3.4.21.62 | -999 |
- |
more |
- |
29427, 29448, 29459, 29480 |
| 3.4.21.62 | -999 |
- |
more |
kinetic analysis of the perhydrolytic wild-type and mutant enzyme activities, overview |
732207 |
| 3.4.21.62 | -999 |
- |
more |
Km of subtilisin Novo chemically attached to soluble DEAE-dextran and insoluble DEAE-Sephadex |
29471 |
| 3.4.21.62 | -999 |
- |
more |
Michaelis-Menten kinetics and thermodynamics |
752829 |
| 3.4.21.62 | -999 |
- |
more |
Michaelis-Menten kinetics using Lineweaver-Burk and Hanes plots |
752524 |
| 3.4.21.62 | -999 |
- |
more |
Michaelis-Menten kinetics using Lineweaver-Burk and Hanes plots at pH 8.5 and 22°C, purified recombinant enzyme |
731172 |
| 3.4.21.62 | -999 |
- |
more |
Michaelis-Menten kinetics, Km is 0.175% w/v |
754435 |
| 3.4.21.62 | -999 |
- |
more |
the temperature dependence of the kinetics and thermodynamic parameters suggest that the enzyme exists in two, i.e. cold and hot forms, at 22°C the cold form turns into the hot one possibly owing to a conformational change |
29458 |
| 3.4.21.62 | 0.000655 |
- |
N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide |
37°C, pH 10.5 |
669738 |
| 3.4.21.62 | 0.0027 |
- |
Suc-Ala-Ala-Pro-Phe-4-nitroanilide |
1 mol lactose bound per 1 mol subtilisin, in 10 mM potassium phosphate buffer, pH 7.8, at 25°C |
707805 |
