General Stability | Organism |
---|---|
Relatively resistant to denaturation by guanidine solutions | Bacillus sp. (in: Bacteria) |
Urea, 4 M, no effect | Bacillus sp. (in: Bacteria) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-(4'-Aminophenylazo)phenylarsonic acid | - |
Bacillus sp. (in: Bacteria) | |
acetone | - |
Bacillus sp. (in: Bacteria) | |
acetonitrile | - |
Bacillus sp. (in: Bacteria) | |
Broad bean extract | subtilisin BPN' | Bacillus sp. (in: Bacteria) | |
Chymotrypsin I inhibitor from potato | subtilisin Carlsberg, subtilisin BPN' | Bacillus sp. (in: Bacteria) | |
Cyclohexanol | - |
Bacillus sp. (in: Bacteria) | |
cyclohexanone | - |
Bacillus sp. (in: Bacteria) | |
DFP | - |
Bacillus sp. (in: Bacteria) | |
Hydrocinnamate | - |
Bacillus sp. (in: Bacteria) | |
indole | - |
Bacillus sp. (in: Bacteria) | |
Inhibitor from egg white | subtilisin Carlsberg, subtilisin BPN' | Bacillus sp. (in: Bacteria) | |
additional information | not: tosyl-leucine chloromethyl ketone | Bacillus sp. (in: Bacteria) | |
p-Arsanilate | - |
Bacillus sp. (in: Bacteria) | |
p-Nitrophenylarsonate | - |
Bacillus sp. (in: Bacteria) | |
p-Tolylarsonate | - |
Bacillus sp. (in: Bacteria) | |
phenol | - |
Bacillus sp. (in: Bacteria) | |
Phenylarsonate | - |
Bacillus sp. (in: Bacteria) | |
phenylmethanesulfonyl fluoride | - |
Bacillus sp. (in: Bacteria) | |
phenylmethanesulfonyl fluoride | - |
Bacillus subtilis | |
Potato extract | subtilisin BPN' | Bacillus sp. (in: Bacteria) | |
tosyl-Phe chloromethyl ketone | not | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7 | - |
benzoyl-Arg ethyl ester | subtilisin Novo, subtilisin Carlsberg | Bacillus sp. (in: Bacteria) | |
10 | - |
benzoyl-Arg ethyl ester | subtilisn BPN' | Bacillus sp. (in: Bacteria) | |
17 | - |
N-acetyl-Phe ethyl ester | subtilisin BPN' | Bacillus sp. (in: Bacteria) | |
22 | - |
N-Acetyl-Tyr ethyl ester | subtilisin BPN' | Bacillus sp. (in: Bacteria) | |
24 | - |
N-Acetyl-Trp ethyl ester | subtilisin BPN' | Bacillus sp. (in: Bacteria) | |
30 | - |
toluenesulfonyl-Arg methyl ester | subtilisin Novo, N-acetyl-Phe methyl ester, subtilisin Carlsberg | Bacillus sp. (in: Bacteria) | |
40 | - |
toluenesulfonyl-Arg methyl ester | subtilisin Carlsberg | Bacillus sp. (in: Bacteria) | |
50 | - |
N-Acetyl-Trp methyl ester | subtilisin Carlsberg | Bacillus sp. (in: Bacteria) | |
60 | - |
N-Acetyl-Phe methyl ester | subtilisin Novo | Bacillus sp. (in: Bacteria) | |
66 | - |
N-Acetyl-Leu methyl ester | subtilisin BPN' | Bacillus sp. (in: Bacteria) | |
70 | - |
N-Acetyl-Tyr ethyl ester | subtilisin Novo, N-acetyl-Tyr methyl ester, subtilisin Carlsberg | Bacillus sp. (in: Bacteria) | |
90 | - |
N-Acetyl-Tyr methyl ester | subtilisin Novo, N-acetyl-Tyr ethyl ester, subtilisin Carlsberg, N-acetyl-Trp methyl ester, subtilisin Novo | Bacillus sp. (in: Bacteria) | |
91 | - |
N-Acetyl-Lys methyl ester | subtilisin BPN' | Bacillus sp. (in: Bacteria) | |
120 | - |
N-Acetyl-Ala methyl ester | subtilisin BPN' | Bacillus sp. (in: Bacteria) |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus subtilis | - |
- |
extracellular | - |
Bacillus sp. (in: Bacteria) | - |
- |
extracellular | - |
Bacillus amyloliquefaciens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme contains no metal ion | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid sequence of: subtilisin BPN' | Bacillus subtilis |
additional information | - |
amino acid sequence of: subtilisin BPN' | Bacillus sp. (in: Bacteria) |
additional information | - |
amino acid sequence of: subtilisin BPN' | Bacillus amyloliquefaciens |
27290 | - |
subtilisin Novo, determination from amino acid composition | Bacillus sp. (in: Bacteria) |
27530 | - |
Bacillus sp., subtilisin BPN', determination from amino acid composition | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus amyloliquefaciens | - |
- |
- |
Bacillus sp. (in: Bacteria) | - |
subtilisin BPN' from Bacillus subtilis, the identity of this strain is later questioned and classified as a species of Bacillus amyloliquefaciens | - |
Bacillus sp. (in: Bacteria) | - |
subtilisin Novo | - |
Bacillus sp. (in: Bacteria) | - |
subtilopeptidase | - |
Bacillus subtilis | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | contains no carbohydrate | Bacillus subtilis |
additional information | contains no carbohydrate | Bacillus sp. (in: Bacteria) |
additional information | contains no carbohydrate | Bacillus amyloliquefaciens |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
overview: isolation procedure | Bacillus sp. (in: Bacteria) |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | - |
Bacillus subtilis | - |
culture medium | - |
Bacillus sp. (in: Bacteria) | - |
culture medium | - |
Bacillus amyloliquefaciens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
assay procedure for esterolytic ,subtilisin Carlsberg or subtilisin BPN', and proteolytic activity | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Benzoyl-Arg ethyl ester + H2O | subtilisin Carlsberg, subtilisin Novo | Bacillus sp. (in: Bacteria) | ? | - |
? | |
Hemoglobin + H2O | denatured | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | reactions catalyzed: 1. peptide bond hydrolysis, 2. ester bond hydrolysis, 3. transesterification, 4. transpeptidation | Bacillus subtilis | ? | - |
? | |
additional information | reactions catalyzed: 1. peptide bond hydrolysis, 2. ester bond hydrolysis, 3. transesterification, 4. transpeptidation | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | reactions catalyzed: 1. peptide bond hydrolysis, 2. ester bond hydrolysis, 3. transesterification, 4. transpeptidation | Bacillus amyloliquefaciens | ? | - |
? | |
N-Acetyl-Ala methyl ester + H2O | subtilisin BPN' | Bacillus sp. (in: Bacteria) | ? | - |
? | |
N-Acetyl-Leu methyl ester + H2O | subtilisin BPN' | Bacillus sp. (in: Bacteria) | ? | - |
? | |
N-Acetyl-Lys methyl ester + H2O | subtilisin BPN' | Bacillus sp. (in: Bacteria) | ? | - |
? | |
N-Acetyl-Phe ethyl ester + H2O | subtilisin BPN' | Bacillus sp. (in: Bacteria) | ? | - |
? | |
N-Acetyl-Phe methyl ester + H2O | subtilisin Carlsberg, subtilisin Novo | Bacillus sp. (in: Bacteria) | ? | - |
? | |
N-Acetyl-Trp ethyl ester + H2O | subtilisin BPN' | Bacillus sp. (in: Bacteria) | ? | - |
? | |
N-Acetyl-Trp methyl ester + H2O | subtilisin Carlsberg, subtilisin Novo | Bacillus sp. (in: Bacteria) | ? | - |
? | |
N-Acetyl-Tyr ethyl ester + H2O | subtilisin Carlsberg, subtilisin Novo | Bacillus sp. (in: Bacteria) | ? | - |
? | |
N-Acetyl-Tyr methyl ester + H2O | subtilisin Carlsberg, subtilisin Novo | Bacillus sp. (in: Bacteria) | ? | - |
? | |
N-Acetyl-Val methyl ester + H2O | subtilisin Carlsberg, subtilisin Novo | Bacillus sp. (in: Bacteria) | ? | - |
? | |
Toluenesulfonyl-Arg methyl ester + H2O | subtilisin Carlsberg, subtilisin Novo | Bacillus sp. (in: Bacteria) | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | synonyms of subtilisin Carlsberg: subtilisin, subtilisin A, subtilopeptidase A, alcalase Novo, synonyms of subtilisin BPN': nagarse proteinase, Nagarse, BPN', subtilopeptidase C, synonyms of subtilisin Novo: bacterial proteinase Novo, subtilisin B, subtilopeptidase B | Bacillus subtilis |
More | synonyms of subtilisin Carlsberg: subtilisin, subtilisin A, subtilopeptidase A, alcalase Novo, synonyms of subtilisin BPN': nagarse proteinase, Nagarse, BPN', subtilopeptidase C, synonyms of subtilisin Novo: bacterial proteinase Novo, subtilisin B, subtilopeptidase B | Bacillus sp. (in: Bacteria) |
More | synonyms of subtilisin Carlsberg: subtilisin, subtilisin A, subtilopeptidase A, alcalase Novo, synonyms of subtilisin BPN': nagarse proteinase, Nagarse, BPN', subtilopeptidase C, synonyms of subtilisin Novo: bacterial proteinase Novo, subtilisin B, subtilopeptidase B | Bacillus amyloliquefaciens |
Subtilisin amylosacchariticus | from Bacillus subtilis var. amylosacchariticus | Bacillus subtilis |
Subtilisin amylosacchariticus | from Bacillus subtilis var. amylosacchariticus | Bacillus sp. (in: Bacteria) |
Subtilisin amylosacchariticus | from Bacillus subtilis var. amylosacchariticus | Bacillus amyloliquefaciens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
rapid denaturation above | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | denatured hemoglobin, subtilisin Novo, subtilisin BPN' | Bacillus sp. (in: Bacteria) |
10 | 11 | casein, subtilisin Carlsberg | Bacillus sp. (in: Bacteria) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
1 | 2 | denaturation | Bacillus sp. (in: Bacteria) |
4 | - |
both active and denatured subtilisins are present simultaneously, rapid autolysis | Bacillus sp. (in: Bacteria) |
11 | - |
above, destabilization of enzyme structure | Bacillus sp. (in: Bacteria) |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for a cofactor | Bacillus sp. (in: Bacteria) |