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EC Number
Inhibitors
Commentary
Structure
1.1.5.2
EDTA
complete inhibition at 0.5 mM
1.1.5.2
ethanol
3.6% residual activity at 80% (v/v)
1.1.5.2
Fe3+
complete inhibition at 10 mM
1.1.5.2
methanol
-
1.1.5.2
methylhydrazine
competitive
1.1.5.2
methylhydrazine
-
1.1.5.2
Mg2+
competitive to Ca2+, Sr2+, or Ba2+, mutants D354N, N355D, and D354N/N355D
1.1.5.2
Mn2+
slight inhibitory effect
1.1.5.2
more
purified enzyme is inactivated upon removal of detergent by acetone treatment. The detergent-depleted enzyme is partially activated by Triton X-100
1.1.5.2
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the protein region responsible for complete EDTA tolerance is located between 32% and 59% from the N-terminus, A27 region
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