EC Number   |
General Stability |
Reference |
|---|
    4.1.1.7 | BFD is stabilized by increasing water activity |
679212 |
| 4.1.1.7 | significant stabilizing effect of thiamin diphosphate and Mg2+ |
655230 |
| 4.1.1.7 | the activity of the spherical silica-immobilized BFD A460I-F464I variant is determined to be 70% related to the activity of the free enzyme |
714487 |
| 4.1.1.7 | The freeze-drying of immobilized Sepabeads EC-EA/BFD leads to an inactivation of the enzyme. |
691346 |
| 4.1.1.7 | the wild type BFD has about 100% enzyme activity after 24 h incubation in 50 mM KPi-buffer without thiamine diphosphate |
716121 |
| 4.1.1.7 | the wild type enzyme shows no loss of activity in cofactor-free buffer over 24 h |
682628 |
| 4.1.1.7 | urea, less than 4 M, reversibly inactivates, thiamin diphosphate protects |
4806 |
