Application | Comment | Organism |
---|---|---|
biotechnology | Production of immobilized enzyme on Sepabeads EC-EA by C-C coupling | Pseudomonas putida |
Cloned (Comment) | Organism |
---|---|
Production as hexahistidine fusion protein by fermentation of Escherichia coli cells as host strain. | Pseudomonas putida |
General Stability | Organism |
---|---|
The freeze-drying of immobilized Sepabeads EC-EA/BFD leads to an inactivation of the enzyme. | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | essential for the activity | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
240000 | - |
- |
Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by Ni-NTA column purification. | Pseudomonas putida |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Pseudomonas putida | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
After 24 h of catalytic activity the immobilized biocatalyst Sepabeads EC-EA/BFD showed a remaining activity of 5% of its initial value. | Pseudomonas putida |
additional information | - |
specific activity of around 62 U/g dry carrier. The activity is determined for the reaction of acetaldehyde (500 mM) and benzaldehyde (50 mM) at pH 8.0 | Pseudomonas putida |
additional information | - |
Stability of Sepabeads EC-EA/BFD is determined by repetitive batch experiments while carrying out the synthesis of 2-hydroxy-1-phenylpropanone. The reaction of benzaldehyde and acetaldehyde is carried out at 30°C and after each batch is washed. The decrease of the initial reaction rate is followed for six batches. The newly prepared biocatalyst (enzyme loading 320 mg/g) shows an initial reaction rate of 130 U/g carrier with respect to the C-C-coupling reaction, whereby the rate decreases down to 10% (13 U/g) in the sixth batch. | Pseudomonas putida |
11 | - |
activity of free BFD | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
benzoylformate decarboxylase | - |
Pseudomonas putida |
BFD | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
at pH 8.0 | Pseudomonas putida |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | At temperatures above 70°C and lower than 20°C the activity decreases below 10 U/g carrier. | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Pseudomonas putida |