Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Escherichia coli SG13009 pKK233-2 | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
H281A | mutant with high cofactor binding affinity | Pseudomonas putida |
L476H | mutant with low cofactor binding affinity | Pseudomonas putida |
L476P | mutant with low cofactor binding affinity | Pseudomonas putida |
L476P/S181T | mutant with low cofactor binding affinity | Pseudomonas putida |
L476Q | the mutant has an intermediate cofactor binding strength | Pseudomonas putida |
S181T | mutant with high cofactor binding affinity | Pseudomonas putida |
General Stability | Organism |
---|---|
the wild type BFD has about 100% enzyme activity after 24 h incubation in 50 mM KPi-buffer without thiamine diphosphate | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzaldehyde + acetaldehyde | - |
Pseudomonas putida | 2-hydroxypropiophenone | - |
? | |
benzoylformate | - |
Pseudomonas putida | benzaldehyde + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BFD | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Pseudomonas putida |