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Information on EC 3.2.1.97 - endo-alpha-N-acetylgalactosaminidase
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EC Tree
3.2.1.97 endo-alpha-N-acetylgalactosaminidaseIUBMB Comments
The enzyme catalyses the liberation of Gal-(1->3)-beta-GalNAc alpha-linked to serine or threonine residues of mucin-type glycoproteins. EngBF from the bacterium Bifidobacterium longum specifically acts on core 1-type O-glycan to release the disaccharide Gal-(1->3)-beta-GalNAc. The enzymes from the bacteria Clostridium perfringens, Enterococcus faecalis, Propionibacterium acnes and Alcaligenes faecalis show broader specificity (e.g. they can also release the core 2 trisaccharide Gal-(1->3)-beta-(GlcNAc-(1->6)-beta)-GalNAc or the core 3 disaccharide GlcNAc-(1->3)-beta-GalNAc) [1,2]. The enzyme may play an important role in the degradation and utilization of mucins having core 1 O-glycan.
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Word Map
- 3.2.1.97
-
o-linked
- neuraminidase
-
o-glycosidic
- n-acetylgalactosamine
- sialidase
-
mucin-type
- asialofetuin
-
o-glycanase
-
bifidobacterial
-
thomsen-friedenreich
- analysis
- galbeta1,3galnac
-
diplococcus
- synthesis
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Reaction Schemes
Synonyms
endo-alpha-n-acetylgalactosaminidase, engcp, engbf, nagbb, spgh101, endo-alpha-galnac-ase, endo-ef, endo-beta-n-acetylgalactosaminidase, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
acetylgalactosaminidase, endo-alpha
-
-
-
-
D-galactosyl-N-acetyl-alpha-D-galactosamine D-galactosyl-N-acetylgalactosaminohydrolase
-
-
-
-
EC 3.2.1.110
-
-
formerly
-
endo-alpha-acetylgalactosaminidase
-
-
-
-
endo-alpha-GalNAc-ase
endo-alpha-N-acetylgalactosaminidase
endo-alpha-N-acetylgalactosaminidase S
endo-EF
endo-GalNAc-ase S
EngBF
EngCP
additional information
endo-alpha-N-acetylgalactosaminidase
-
-
-
-
additional information
-
the enzyme belongs to the glycoside hydrolase family 101, GH101
additional information
the enzyme belongs to the glycoside hydrolase family 101, GH101
additional information
-
the enzyme belongs to the glycoside hydrolase family 101, GH101
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-[glycoprotein]-L-serine/L-threonine + H2O = beta-D-galactosyl-(1->3)-N-acetyl-D-galactosamine + [glycoprotein]-L-serine/L-threonine
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
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-
-
-
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SYSTEMATIC NAME
IUBMB Comments
glycopeptide-D-galactosyl-N-acetyl-alpha-D-galactosamine D-galactosyl-N-acetyl-galactosaminohydrolase
The enzyme catalyses the liberation of Gal-(1->3)-beta-GalNAc alpha-linked to serine or threonine residues of mucin-type glycoproteins. EngBF from the bacterium Bifidobacterium longum specifically acts on core 1-type O-glycan to release the disaccharide Gal-(1->3)-beta-GalNAc. The enzymes from the bacteria Clostridium perfringens, Enterococcus faecalis, Propionibacterium acnes and Alcaligenes faecalis show broader specificity (e.g. they can also release the core 2 trisaccharide Gal-(1->3)-beta-(GlcNAc-(1->6)-beta)-GalNAc or the core 3 disaccharide GlcNAc-(1->3)-beta-GalNAc) [1,2]. The enzyme may play an important role in the degradation and utilization of mucins having core 1 O-glycan.
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CAS REGISTRY NUMBER
COMMENTARY
59793-96-3
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2,4-dinitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc + H2O
2,4-dinitrophenol + beta-D-Gal-(1->3)-alpha-D-GalNAc
-
-
-
?
2,4-dinitrophenyl-GalNAc-alpha-R + H2O
2,4-dinitrophenol + GalNAc-alpha-R
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
4,6-dimethoxy-1,3,5-triazin-2-yl alpha-N-acetylgalactosaminide + H2O
?
-
-
-
?
4-nitrophenyl 2-(acetylamino)-2-deoxy-alpha-D-galactopyranoside + H2O
4-nitrophenol + N-acetyl-alpha-D-galactosamine
4-nitrophenyl 2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside + H2O
4-nitrophenol + 3-O-beta-D-galactopyranosyl-2-acetamido-2-deoxy-alpha-D-galactopyranose
-
-
-
-
?
4-nitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc + H2O
4-nitrophenol + beta-D-Gal-(1->3)-alpha-D-GalNAc
4-nitrophenyl-N-acetyl-beta-D-galactosaminide + H2O
4-nitrophenol + N-acetyl-beta-D-galactosamine
-
-
-
?
4-nitrophenyl-N-acetyl-beta-D-galactosaminide + L-Ser
?
transglycosylation
-
-
?
asialo-fetuin + H2O
?
-
almost complete removal of O-linked Galbeta1-3GalNAc, the product has a decreased trypsin inhibitory activity
-
?
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + 1-butanol
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-butyl + p-nitrophenol
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + 1-heptanol
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-heptyl + p-nitrophenol
-
transfer ratio in presence of sodium cholate is 22.7%
-
?
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + 1-hexanol
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-hexyl + p-nitrophenol
-
transfer ratio in presence of sodium cholate is 58.8%
-
?
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + 1-octanol
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-1-octyl + p-nitrophenol
-
transfer ratio in presence of sodium cholate is 14.9%
-
?
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + 1-pentanol
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-pentyl + p-nitrophenol
-
transfer ratio in presence of sodium cholate is 73.3%
-
?
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + 1-propanol
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-propyl + p-nitrophenol
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + 2-benzyloxy-1-pentanol
?
-
transfer ratio in presence of sodium cholate is 56.3%
-
?
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + 2-benzyloxyethanol
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-(2-benzyloxyethyl) + p-nitrophenol
-
transfer ratio in presence of sodium cholate is 80.8%
-
?
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + allyl alcohol
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-allyl + p-nitrophenol
-
transfer ratio in presence of sodium cholate is 86.8%
-
?
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + benzyloxycarbonyl-Leu-Ser-Gln-Val-His-Arg
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-(CBZ-Leu)-Ser-Gln-Val-His-Arg + p-nitrophenol
-
-
-
?
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + diethylene glycol monomethyl ether
?
-
transfer ratio in presence of sodium cholate is 78.6%
-
?
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + ethanol
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-ethyl + p-nitrophenol
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + ethylene glycol monomethyl ether
?
-
transfer ratio in presence of sodium cholate is 79.4%
-
?
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + methanol
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-methyl + p-nitrophenol
Galbeta(1,3)GalNAc-asialofetuin + H2O
Galbeta(1,3)GalNAc + asialofetuin
pH 5.0, 37°C
-
-
?
Galbeta(1-3)GalNAcalpha(1-)OC6H4-o-NO2 + H2O
Galbeta(1-3)GalNAc + o-nitrophenol
-
-
-
-
?
Galbeta(1-3)GalNAcalpha(1-)OC6H4-p-NO2 + H2O
Galbeta(1-3)GalNAc + p-nitrophenol
-
-
-
-
?
Galbeta(1-3)GalNAcalpha(1-)OC6H5 + H2O
Galbeta(1-3)GalNAc + phenol
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-
-
-
?
Galbeta(1-3)GalNAcalpha(1-)p-nitrophenyl + galactose
?
-
transglycosylation
-
-
?
Galbeta(1-3)GalNAcalpha(1-)p-nitrophenyl + glucose
?
-
transglycosylation
-
-
?
Galbeta(1-3)GalNAcalpha(1-)p-nitrophenyl + H2O
Galbeta(1-3)GalNAc + p-nitrophenol
-
-
-
-
?
Galbeta(1-3)GalNAcalpha(1-)p-nitrophenyl + maltose
?
-
transglycosylation
-
-
?
Galbeta(1-3)GalNAcalpha(1-)p-nitrophenyl + mannitol
?
-
transglycosylation
-
-
?
Galbeta(1-3)GalNAcalpha(1-)p-nitrophenyl + mannose
?
-
transglycosylation
-
-
?
Galbeta(1-3)GalNAcalpha(1-)p-nitrophenyl + sorbitol
?
-
transglycosylation
-
-
?
Galbeta(1-3)GalNAcalpha(1-)p-nitrophenyl + sucrose
?
-
transglycosylation
-
-
?
Galbeta(1-3)GalNAcalpha1-p-nitrophenol + H2O
Galbeta(1-3)GalNAc + p-nitrophenol
-
-
-
?
Tn antigen + H2O
?
i.e. N-acetyl-beta-D-galactosaminyl-L-Ser/Thr
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
-
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
-
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
-
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
-
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
-
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
-
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-serine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-serine-[protein]
-
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
-
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
-
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
-
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
-
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
-
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
-
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
-
-
-
?
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosaminyl-L-threonine-[protein] + H2O
3-O-beta-D-galactosyl-N-acetyl-alpha-D-galactosamine + L-threonine-[protein]
-
-
-
-
?
4-nitrophenyl 2-(acetylamino)-2-deoxy-alpha-D-galactopyranoside + H2O
4-nitrophenol + N-acetyl-alpha-D-galactosamine
1.8% relative activity
-
-
?
4-nitrophenyl 2-(acetylamino)-2-deoxy-alpha-D-galactopyranoside + H2O
4-nitrophenol + N-acetyl-alpha-D-galactosamine
1.8% relative activity
-
-
?
4-nitrophenyl 2-(acetylamino)-2-deoxy-alpha-D-galactopyranoside + H2O
4-nitrophenol + N-acetyl-alpha-D-galactosamine
-
-
-
?
4-nitrophenyl 2-(acetylamino)-2-deoxy-alpha-D-galactopyranoside + H2O
4-nitrophenol + N-acetyl-alpha-D-galactosamine
-
-
-
?
4-nitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc + H2O
4-nitrophenol + beta-D-Gal-(1->3)-alpha-D-GalNAc
-
-
-
-
?
4-nitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc + H2O
4-nitrophenol + beta-D-Gal-(1->3)-alpha-D-GalNAc
-
-
-
-
?
4-nitrophenyl beta-D-Gal-(1->3)-alpha-D-GalNAc + H2O
4-nitrophenol + beta-D-Gal-(1->3)-alpha-D-GalNAc
-
-
-
?
asialoglycophorin + H2O
Galbeta(1-3)GalNAc + ?
-
asialoglycophorin A
-
-
?
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + 1-butanol
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-butyl + p-nitrophenol
-
transfer ratio in presence of sodium cholate is 85.6%
-
?
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + 1-butanol
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-butyl + p-nitrophenol
-
transfer ratio in presence of sodium cholate is 85.6%
-
?
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + 1-propanol
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-propyl + p-nitrophenol
-
transfer ratio in presence of sodium cholate is 76.8%
-
?
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + 1-propanol
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-propyl + p-nitrophenol
-
transfer ratio in presence of sodium cholate is 76.8%
-
?
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + ethanol
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-ethyl + p-nitrophenol
-
transfer ratio in presence of sodium cholate is 65.0%
-
?
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + ethanol
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-ethyl + p-nitrophenol
-
transfer ratio in presence of sodium cholate is 65.0%
-
?
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + methanol
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-methyl + p-nitrophenol
-
transfer ratio in presence of sodium cholate is 70.8%
-
?
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + methanol
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-methyl + p-nitrophenol
-
transfer ratio in presence of sodium cholate is 70.8%
-
?
fetuin + H2O
?
the enzyme catalyzes the cleavage of core 1 and core 3 type O-linked disaccharides between GalNAc and serine or threonine residues from glycoproteins
-
-
?
fetuin + H2O
?
-
hydrolysis of the O-glycosidic linkage between GalNAc and Ser or Thr
-
-
?
fetuin + H2O
?
-
hydrolysis of the O-glycosidic linkage between GalNAc and Ser or Thr
-
-
?
Gal-beta-1,3-GalNAc-alpha-1p-nitrophenol + H2O
p-nitrophenol + Gal-beta-1,3-GalNAc
pH 5.0, 37°C
-
-
?
Gal-beta-1,3-GalNAc-alpha-1p-nitrophenol + H2O
p-nitrophenol + Gal-beta-1,3-GalNAc
-
pH 5.0, 37°C
-
-
?
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol + H2O
p-nitrophenol + Galbeta(1,3)GalNAc
-
pH 5.0, 37°C
-
-
?
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol + H2O
p-nitrophenol + Galbeta(1,3)GalNAc
-
pH 5.0, 37°C
-
-
?
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol + H2O
p-nitrophenol + Galbeta(1,3)GalNAc
-
pH 5.0, 37°C
-
-
?
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol + H2O
p-nitrophenol + Galbeta(1,3)GalNAc
-
pH 5.0, 37°C
-
-
?
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol + H2O
p-nitrophenol + Galbeta(1,3)GalNAc
-
pH 5.0, 37°C
-
-
?
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol + H2O
p-nitrophenol + Galbeta(1,3)GalNAc
pH 5.0, 37°C
-
-
?
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol + H2O
p-nitrophenol + Galbeta(1,3)GalNAc
pH 5.0, 37°C
-
-
?
Galbeta(1,3)GalNAcalpha-1-p-nitrophenol + H2O
p-nitrophenol + Galbeta(1,3)GalNAc
pH 5.0, 37°C
-
-
?
Galbeta1-3(GlcNAcbeta1-6)GalNAcalpha1-pNP + H2O
?
2.3% relative activity
-
-
?
Galbeta1-3(GlcNAcbeta1-6)GalNAcalpha1-pNP + H2O
?
2.3% relative activity
-
-
?
Galbeta1-3GalNAcalpha-pNP + H2O
Galbeta1-3GalNAc + p-nitrophenol
-
-
-
?
Galbeta1-3GalNAcalpha-pNP + H2O
Galbeta1-3GalNAc + p-nitrophenol
-
-
-
?
Galbeta1-3GalNAcalpha1-pNP + H2O
?
100% relative activity
-
-
?
Galbeta1-3GalNAcalpha1-pNP + H2O
?
100% relative activity
-
-
?
Galbeta1-3[Galbeta1-3GlcNAcbeta1-6]GalNAcalpha-pNP + H2O
?
-
-
-
?
Galbeta1-3[Galbeta1-3GlcNAcbeta1-6]GalNAcalpha-pNP + H2O
?
-
-
-
?
Galbeta1-3[GlcNAcbeta1-6]GalNAcalpha1-pNP + H2O
?
-
-
-
?
GalNAcbeta1-3GalNAcalpha1-pNP + H2O
?
21% relative activity
-
-
?
gastric mucin + H2O
disaccharide + ?
-
EngBF does not release oligosaccharides from intact gastric mucin, but releases core 1 disaccharide from the gastric mucin pretreated with commercial sialidase and with the recombinant exo-alpha-N-acetylglucosamindase
-
-
?
gastric mucin + H2O
disaccharide + ?
EngCP does not release oligosaccharides from intact gastric mucin, but releases core 1 disaccharide from the gastric mucin pretreated with commercial sialidase and with the recombinant exo-alpha-N-acetylglucosamindase cloned from the same strain of Clostridium perfringens. It does not release core 2 trisaccharide from the mucin
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Glcbeta1-3GalNAcalpha1-pNP + H2O
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156.7% relative activity
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?
GlcNAcbeta1-3GalNAcalpha1-pNP + H2O
?
3.5% relative activity
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glycopeptide + H2O
Galbeta(1-3)GalNAc + ?
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specific requirement for an unsubstituted galactose at the nonreducing terminus and an alpha-linkage between N-acetylgalactosamine and the aglycone
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?
glycopeptide + H2O
Galbeta(1-3)GalNAc + ?
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glycopeptides from mouse myeloma, fetuin and pig submaxillary mucin
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?
glycopeptide + H2O
Galbeta(1-3)GalNAc + ?
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release of Galbeta(1-3)GalNAc from glycopeptides possesing Ser or Thr O-glycosidic linkages
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?
glycoprotein + H2O
Galbeta(1-3)GalNAc + ?
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release of Galbeta(1-3)GalNAc from glycoproteins possesing Ser or Thr O-glycosidic linkages
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?
glycoprotein + H2O
Galbeta(1-3)GalNAc + ?
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release of Galbeta(1-3)GalNAc from glycoproteins possesing Ser or Thr O-glycosidic linkages
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?
glycoprotein + H2O
Galbeta(1-3)GalNAc + ?
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hydrolyzes the O-glycosidic linkage between alpha-N-acetylgalactosamine and the hydroxyl group of Ser or Thr residues in mucus and mucin-type glycoproteins
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?
glycoprotein + H2O
Galbeta(1-3)GalNAc + ?
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hydrolyzes the O-glycosidic linkage between alpha-N-acetylgalactosamine and the hydroxyl group of Ser or Thr residues in mucus and mucin-type glycoproteins
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?
glycoprotein + H2O
Galbeta(1-3)GalNAc + ?
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digestion of nerve cell surface glycoproteins or their terminal N-acetylgalactosamines, no digestion of galactosamines associated with the collagenous ligands or perineuronal proteoglycan
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?
glycoprotein + H2O
Galbeta(1-3)GalNAc + ?
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specific requirement for an unsubstituted galactose in the nonreducing terminus and an alpha-linkage between N-acetylgalactosamine and the aglycone
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?
glycoprotein + H2O
Galbeta(1-3)GalNAc + ?
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disialized human erythrocyte membrane glycoprotein, porcine submaxillarry mucin
no oligosaccharides larger than trisaccharides are liberated from porcine submaxillarry mucin
?
glycoprotein + H2O
Galbeta(1-3)GalNAc + ?
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bovine submaxillary mucin, no hydrolysis of remodelled antifreeze glycoprotein containing NeuAcalpha2-3Galbeta1-3GalNAc and Galbeta1-3(NeuAcalpha2-6)GalNAc
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?
glycoprotein + H2O
Galbeta(1-3)GalNAc + ?
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release of Galbeta(1-3)GalNAc from glycoproteins possesing Ser or Thr O-glycosidic linkages
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?
glycoprotein + H2O
Galbeta(1-3)GalNAc + ?
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release of Galbeta(1-3)GalNAc from glycoproteins possesing Ser or Thr O-glycosidic linkages
no oligosaccharides larger than trisaccharides are liberated from porcine submaxillarry mucin
?
glycoprotein + H2O
Galbeta(1-3)GalNAc + ?
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liberates Galbeta(1-3)4GalNAc and also other larger oligosaccharides by hydrolysis of O-glycosidic linkage between GalNAc and Ser or Thr
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?
glycoprotein + H2O
Galbeta(1-3)GalNAc + ?
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purified pig gastric mucus glycoproteins
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?
glycoprotein + H2O
Galbeta(1-3)GalNAc + ?
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liberates Galbeta(1-3)4GalNAc and also other larger oligosaccharides by hydrolysis of O-glycosidic linkage between GalNAc and Ser or Thr
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?
glycoprotein + H2O
Galbeta(1-3)GalNAc + ?
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purified pig gastric mucus glycoproteins
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?
mucin + H2O
Galbeta1-3GalNAc + ?
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desialylated bovine submaxillary mucin
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?
mucin + H2O
Galbeta1-3GalNAc + ?
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release of Galbeta(1-3)GalNAc from mucins possesing Ser or Thr O-glycosidic linkages
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?
mucin + H2O
Galbeta1-3GalNAc + ?
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release of Galbeta(1-3)GalNAc from mucins possesing Ser or Thr O-glycosidic linkages
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?
asialofetuin + H2O
additional information
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Galbeta(1-3)GalNAc + ?
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asialofetuin + H2O
additional information
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Galbeta(1-3)GalNAc + ?
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asialofetuin + H2O
additional information
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Galbeta(1-3)GalNAc + ?
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asialofetuin + H2O
additional information
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hydrolysis of the O-glycosidic linkage between GalNAc and Ser or Thr
Galbeta(1-3)GalNAc + Galbeta(1-3)(Galbeta(1-4)GlcNAcbeta(1-6))GalNAc
?
asialofetuin + H2O
additional information
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hydrolysis of the O-glycosidic linkage between GalNAc and Ser or Thr
Galbeta(1-3)GalNAc + Galbeta(1-3)(Galbeta(1-4)GlcNAcbeta(1-6))GalNAc
?
additional information
?
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highly induced by porcine gastric mucin
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?
additional information
?
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the enzyme also catalyzes the transfer of beta-D-Gal-(1-3)-alpha-D-GalNAc from asialofetuin to 1-alkanols
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?
additional information
?
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the enzyme also catalyzes the transfer of beta-D-Gal-(1-3)-alpha-D-GalNAc from asialofetuin to 1-alkanols
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?
additional information
?
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the expression of the gene is highly induced in the presence of mucin
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?
additional information
?
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the expression of the gene is highly induced in the presence of mucin
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?
additional information
?
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the enzyme acts very weakly on GalNAcalpha1-UDP and GalNAcalpha(1>3)Galbeta(1>4)Glc and not at all on Neu5Acalpha(2>6)GalNAcalpha1-L-Ser (sialyl Tn antigen)and GalNAcalpha(1>3)(Fucalpha(1>2))Gal (blood group A trisaccharide). In addition, the enzyme very slowly hydrolyzes core-3 (4-nitrophenyl-GlcNAcbeta(1>3)GalNAcalpha1) and core-8 (4-nitrophenyl-Galalpha(1>3)GalNAcalpha1)-type structures but not other core structures
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?
additional information
?
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Galbeta(1,3)GalNAc disaccharide is liberated from asialofetuin and p-nitrophenol substrate containing the core 1 structure (Galbeta(1,3)GalNAcalpha-1), but not from sialofetuin or any p-nitrophenol substrate other than Galbeta(1,3)GalNAcalpha-1-p-nitrophenol
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?
additional information
?
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Galbeta(1,3)GalNAc disaccharide is liberated from asialofetuin and p-nitrophenol substrate containing the core 1 structure (Galbeta(1,3)GalNAcalpha-1), but not from sialofetuin or any p-nitrophenol substrate other than Galbeta(1,3)GalNAcalpha-1-p-nitrophenol
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?
additional information
?
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no reaction with Galbeta(1,3)(GlcNAcbeta(1,6))GalNAcalpha-1-p-nitrophenol, GalNAcalpha-1-p-nitrophenol, GlcNAcbeta(1,3)GalNAcalpha-1-p-nitrophenol and Galbeta(1,3)GlcNAcalpha-1-p-nitrophenol
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?
additional information
?
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no reaction with Galbeta(1,3)(GlcNAcbeta(1,6))GalNAcalpha-1-p-nitrophenol, GalNAcalpha-1-p-nitrophenol, GlcNAcbeta(1,3)GalNAcalpha-1-p-nitrophenol and Galbeta(1,3)GlcNAcalpha-1-p-nitrophenol
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?
additional information
?
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the enzyme also exhibits transglycosylation activity towards various mono- and disaccharides and 1-alkanols. No activity with GalNAc-p-nitrophenol, Galbeta(1-3)(GlcNAcbeta(1-6))GalNAc-p-nitrophenol, GlcNAcbeta(1-3)GalNAc-p-nitrophenol, Galbeta(1-3)GlcNAc-p-nitrophenol. Does not release sialo-oligosaccharides from fetuin
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?
additional information
?
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the enzyme also exhibits transglycosylation activity towards various mono- and disaccharides and 1-alkanols. No activity with GalNAc-p-nitrophenol, Galbeta(1-3)(GlcNAcbeta(1-6))GalNAc-p-nitrophenol, GlcNAcbeta(1-3)GalNAc-p-nitrophenol, Galbeta(1-3)GlcNAc-p-nitrophenol. Does not release sialo-oligosaccharides from fetuin
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?
additional information
?
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EngCP from Clostridium perfringens possesses broader substrate specificity than EngBF
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?
additional information
?
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EngBF preferably releases Galbeta1-3GalNAc from the core 1-type O-glycan, i.e. Thomsen-Friedenreich antigen or T-antigen, of mucin glycoproteins. EngBF also shows transglycosylation activity of the released disaccharide to other mono- and disaccharides
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?
additional information
?
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the enzyme hydrolyses the O-glycosidic bonds in mucin-type O-glycan between alpha-GalNAc and Ser/Thr. EngBF is highly specific for the core 1-type O-glycan to release the disaccharide Galbeta1-3GalNAc
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?
additional information
?
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active site structure and substrate binding by the enzyme, important residues for substrate binding are Trp residues Trp748 and Trp750, appearing to form stacking interactions with the beta-faces of sugar rings of Galbeta1-3GalNAc by substrate-induced fit, substrate specificity for glycans, docking analysis, detailed overview
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?
additional information
?
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endo-alpha-N-acetylgalactosaminidase catalyzes the release of Galbeta1-3GalNAc from the core 1-type O-glycan, Galbeta1-3GalNAcalpha1-Ser/Thr, of mucin glycoproteins and synthetic 4-nitrophenyl alpha-linked substrates. The enzyme directly transfers Galbeta1-3GalNAc to serine or threonine residues of bioactive peptides such as PAMP-12, bradykinin, peptide-TandMUC1a when Galbeta1-3GalNAcalpha1-4NP was used as a donor substrate. The enzyme also catalyzes the reverse-hydrolysis reaction. EngBF synthesizes the core 1 disaccharide-containing oligosaccharides when the enzyme is incubated with either glucose or lactose and