Cloned (Comment) | Organism |
---|---|
into vector pET23a and expressed with a histidine-tag in Escherichia coli BL21(DE3) | Enterococcus faecalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM greatly inhibits | Enterococcus faecalis | |
additional information | 1 mM of Mg2+, Ni2+, Ca2+, Co2+, Na+, Mn2+, Fe2+, Fe3+, EGTA, and EDTA have little effect on the enzyme activity | Enterococcus faecalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0596 | - |
Galbeta1-3GalNAcalpha-pNP | - |
Enterococcus faecalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
150000 | - |
1 * 150000, SDS-PAGE | Enterococcus faecalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | Q833X5 | - |
- |
Enterococcus faecalis NBRC3971 | Q833X5 | - |
- |
Purification (Comment) | Organism |
---|---|
by gel filtration | Enterococcus faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl 2-(acetylamino)-2-deoxy-alpha-D-galactopyranoside + H2O | - |
Enterococcus faecalis | 4-nitrophenol + N-acetyl-alpha-D-galactosamine | - |
? | |
4-nitrophenyl 2-(acetylamino)-2-deoxy-alpha-D-galactopyranoside + H2O | - |
Enterococcus faecalis NBRC3971 | 4-nitrophenol + N-acetyl-alpha-D-galactosamine | - |
? | |
Galbeta1-3GalNAcalpha-pNP + H2O | - |
Enterococcus faecalis | Galbeta1-3GalNAc + p-nitrophenol | - |
? | |
Galbeta1-3GalNAcalpha-pNP + H2O | - |
Enterococcus faecalis NBRC3971 | Galbeta1-3GalNAc + p-nitrophenol | - |
? | |
Galbeta1-3[Galbeta1-3GlcNAcbeta1-6]GalNAcalpha-pNP + H2O | - |
Enterococcus faecalis | ? | - |
? | |
Galbeta1-3[Galbeta1-3GlcNAcbeta1-6]GalNAcalpha-pNP + H2O | - |
Enterococcus faecalis NBRC3971 | ? | - |
? | |
Galbeta1-3[GlcNAcbeta1-6]GalNAcalpha1-pNP + H2O | - |
Enterococcus faecalis | ? | - |
? | |
Galbeta1-3[GlcNAcbeta1-6]GalNAcalpha1-pNP + H2O | - |
Enterococcus faecalis NBRC3971 | ? | - |
? | |
Glc-NAcbeta1-3GalNAcalpha1-pNP + H2O | - |
Enterococcus faecalis | ? | - |
? | |
additional information | can not release the oligosaccharide from GlcNAcbeta1-6GalNAcalpha1-pNP | Enterococcus faecalis | ? | - |
? | |
additional information | can not release the oligosaccharide from GlcNAcbeta1-6GalNAcalpha1-pNP | Enterococcus faecalis NBRC3971 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 150000, SDS-PAGE | Enterococcus faecalis |
Synonyms | Comment | Organism |
---|---|---|
endo-alpha-N-acetylgalactosaminidase | - |
Enterococcus faecalis |
endo-EF | - |
Enterococcus faecalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Enterococcus faecalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
49.9 | - |
Galbeta1-3GalNAcalpha-pNP | - |
Enterococcus faecalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Enterococcus faecalis |