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EC Tree
The expected taxonomic range for this enzyme is: Eukaryota, Archaea, Bacteria
Synonyms
GDP-glucosepyrophosphorylase, GDPG pyrophosphorylase, GDPglucose pyrophosphorylase, glucose 1-phosphate guanylyltransferase, guanosine diphosphate glucose pyrophosphorylase, guanosine diphosphoglucose pyrophosphorylase, guanylyltransferase, glucose 1-phosphate, Man-1-PGTase, mannose-1-phosphate guanylyltransferase, More,
more
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GDP-glucosepyrophosphorylase
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GDPG pyrophosphorylase
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GDPglucose pyrophosphorylase
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glucose 1-phosphate guanylyltransferase
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guanosine diphosphate glucose pyrophosphorylase
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guanosine diphosphoglucose pyrophosphorylase
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guanylyltransferase, glucose 1-phosphate
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mannose-1-phosphate guanylyltransferase
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multiple sugar-1-P NTase
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additional information
cf. EC 2.7.7.37 and EC 2.7.7.13
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GTP + alpha-D-glucose 1-phosphate = diphosphate + GDP-glucose
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nucleotidyl group transfer
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GTP:alpha-D-glucose-1-phosphate guanylyltransferase
Also acts, more slowly, on D-mannose 1-phosphate.
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dGTP + alpha-D-glucosamine 1-phosphate
diphosphate + dGDP-glucosamine
very low activity
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dGTP + alpha-D-glucose 1-phosphate
diphosphate + dGDP-glucose
very low activity
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GTP + alpha-D-glucosamine 1-phosphate
diphosphate + GDP-glucosamine
best substrate
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?
GTP + alpha-D-glucose 1-phosphate
diphosphate + GDP-glucose
GTP + D-mannose 1-phosphate
diphosphate + GDP-mannose
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less effective than alpha-D-glucose 1-phosphate
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r
additional information
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GTP + alpha-D-glucose 1-phosphate
diphosphate + GDP-glucose
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r
GTP + alpha-D-glucose 1-phosphate
diphosphate + GDP-glucose
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?
GTP + alpha-D-glucose 1-phosphate
diphosphate + GDP-glucose
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r
GTP + alpha-D-glucose 1-phosphate
diphosphate + GDP-glucose
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r
GTP + alpha-D-glucose 1-phosphate
diphosphate + GDP-glucose
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r
GTP + alpha-D-glucose 1-phosphate
diphosphate + GDP-glucose
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GTP + alpha-D-glucose 1-phosphate
diphosphate + GDP-glucose
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r
GTP + alpha-D-glucose 1-phosphate
diphosphate + GDP-glucose
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r
GTP + alpha-D-glucose 1-phosphate
diphosphate + GDP-glucose
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r
GTP + alpha-D-glucose 1-phosphate
diphosphate + GDP-glucose
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GTP + alpha-D-glucose 1-phosphate
diphosphate + GDP-glucose
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GTP + alpha-D-glucose 1-phosphate
diphosphate + GDP-glucose
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r
additional information
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ATP, CTP, TTP, UTP are ineffective
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additional information
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involved in biosynthesis of heparin in mast cell tissue
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additional information
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a bifunctional enzyme with phosphomannose isomerase (PMI), EC 5.3.1.8, and mannose-1-phosphate guanylyltransferase (Man-1-P GTase), EC 2.7.7.13, activities, which can synthesize GDP-mannose when accompanied by a phosphomannomutase/phosphoglucomutase (PMM/PGM) enzyme (PH0923). PH0925 protein is a thermostable enzyme with both PMI and multiple sugar-1-P NTase, cf. EC 2.7.7.37 and EC 2.7.7.34, activities
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additional information
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substrate specificity of the sugar-1-P NTase activity of the PH0925 protein, overview. No activity with fructose-1-phosphate, alpha-D-galactose-1-phosphate, and N-acetyl-D-glucosamine-1-phosphate and no activity with ATP, CTP, UTP, dATP, dCTP, and dTTP
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additional information
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involved in microbial metabolism of guanosine sugar nucleotides in Streptomyces sp.
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additional information
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involved in microbial metabolism of guanosine sugar nucleotides in Streptomyces sp.
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additional information
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involved in microbial metabolism of guanosine sugar nucleotides in Streptomyces sp.
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additional information
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involved in microbial metabolism of guanosine sugar nucleotides in Streptomyces sp.
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additional information
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involved in microbial metabolism of guanosine sugar nucleotides in Streptomyces sp.
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additional information
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involved in microbial metabolism of guanosine sugar nucleotides in Streptomyces sp.
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additional information
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involved in microbial metabolism of guanosine sugar nucleotides in Streptomyces sp.
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additional information
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involved in microbial metabolism of guanosine sugar nucleotides in Streptomyces sp.
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additional information
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involved in microbial metabolism of guanosine sugar nucleotides in Streptomyces sp.
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additional information
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involved in microbial metabolism of guanosine sugar nucleotides in Streptomyces sp.
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additional information
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additional information
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involved in biosynthesis of heparin in mast cell tissue
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additional information
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a bifunctional enzyme with phosphomannose isomerase (PMI), EC 5.3.1.8, and mannose-1-phosphate guanylyltransferase (Man-1-P GTase), EC 2.7.7.13, activities, which can synthesize GDP-mannose when accompanied by a phosphomannomutase/phosphoglucomutase (PMM/PGM) enzyme (PH0923). PH0925 protein is a thermostable enzyme with both PMI and multiple sugar-1-P NTase, cf. EC 2.7.7.37 and EC 2.7.7.34, activities
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additional information
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involved in microbial metabolism of guanosine sugar nucleotides in Streptomyces sp.
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additional information
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involved in microbial metabolism of guanosine sugar nucleotides in Streptomyces sp.
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additional information
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involved in microbial metabolism of guanosine sugar nucleotides in Streptomyces sp.
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additional information
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involved in microbial metabolism of guanosine sugar nucleotides in Streptomyces sp.
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additional information
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involved in microbial metabolism of guanosine sugar nucleotides in Streptomyces sp.
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additional information
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involved in microbial metabolism of guanosine sugar nucleotides in Streptomyces sp.
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additional information
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involved in microbial metabolism of guanosine sugar nucleotides in Streptomyces sp.
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additional information
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involved in microbial metabolism of guanosine sugar nucleotides in Streptomyces sp.
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additional information
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involved in microbial metabolism of guanosine sugar nucleotides in Streptomyces sp.
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additional information
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involved in microbial metabolism of guanosine sugar nucleotides in Streptomyces sp.
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Cu2+
best activating metal ion
additional information
the metal cofactor requirements of the multifunctional enzyme differ with the substrates used, overview
Mg2+
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absolute requirement, highest activity at 8-10 mM
Mg2+
highly activates sugar-1-P NTase activity with substrate Man-1-P, only slightly activating with substrates Glc-1-P and GlcN-1-P
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D-mannose 1-phosphate
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0.07 mM: 18% inhibition in presence of 3.5 mM GDPglucose, 40% inhibition in presence of 0.7 mM GDPglucose, no inhibition in presence of more than 7 mM GDPglucose
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0.8
diphosphate
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pH 7.4, 30°C
0.1
GDPglucose
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pH 7.4, 30°C
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0.2
with dGTP and alpha-D-glucose 1-phosphate, purified His-tagged recombinant enzyme, pH 7.6, 85°C
0.5
with dGTP and alpha-D-glucosamine 1-phosphate, purified His-tagged recombinant enzyme, pH 7.6, 85°C
5.2
with GTP and alpha-D-glucose 1-phosphate, purified His-tagged recombinant enzyme, pH 7.6, 85°C
7.3
with GTP and alpha-D-glucosamine 1-phosphate, purified His-tagged recombinant enzyme, pH 7.6, 85°C
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6 - 8.7
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pH 6: 65% of maximal activity, pH 8.7: 60% of maximal activity at pH 8.7
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mast cell tumor, grown in LAF mice
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GenBank
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GenBank
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IFO 3439, IFO 3123
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IFO 3122, IFO 3430, IFO 3356
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IFO 3145
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IFO 3409
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IFO 3389
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IFO 3441
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IFO 3310
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IFO 3111
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additional information
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no activity in fibrosarcomase and umbilical cord
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evolution
the enzyme belongs to the mannose-6-phosphate isomerase type 2 family
evolution
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the enzyme belongs to the mannose-6-phosphate isomerase type 2 family
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evolution
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the enzyme belongs to the mannose-6-phosphate isomerase type 2 family
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additional information
the sugar-1-phosphate nucleotidylyltransferase activity is located in the region from the N-terminus to the 345th residue, the Man-1-P GTase activity is located in the C-terminal 114 residue region of the PH0925 protein, the phosphomannose isomerase requires the the C-terminal 14 residues
additional information
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the sugar-1-phosphate nucleotidylyltransferase activity is located in the region from the N-terminus to the 345th residue, the Man-1-P GTase activity is located in the C-terminal 114 residue region of the PH0925 protein, the phosphomannose isomerase requires the the C-terminal 14 residues
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additional information
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the sugar-1-phosphate nucleotidylyltransferase activity is located in the region from the N-terminus to the 345th residue, the Man-1-P GTase activity is located in the C-terminal 114 residue region of the PH0925 protein, the phosphomannose isomerase requires the the C-terminal 14 residues
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O58649_PYRHO
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
464
0
52890
TrEMBL
other Location (Reliability: 5 )
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additional information
construction of several truncated mutants of the PH0925 protein, that show altered kinetics compared to the wild-type enzyme, overview
additional information
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construction of several truncated mutants of the PH0925 protein, that show altered kinetics compared to the wild-type enzyme, overview
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additional information
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construction of several truncated mutants of the PH0925 protein, that show altered kinetics compared to the wild-type enzyme, overview
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-20°C, dried acetone powder, stable for at least two weeks
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mast cell tumor, grown in LAF mice, partial
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recombinant His-tagged PH0925 protein from Escherichia coli strain BL21-Codon Plus(DE3)-RIL by nickel affinity chromatography
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gene PH0925, recombinant expression of His-tagged enzyme PH0925 in Escherichia coli strain BL21-Codon Plus(DE3)-RIL
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Danishefsky, I.; Heritier-Watkins, O.
Nucleoside diphosphate glucose pyrophosphorylases in mast cell tumors
Biochim. Biophys. Acta
139
349-357
1967
Mus musculus
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Kawaguchi, K.; Tanida, S.; Matsuda, K.; Tani, Y.; Ogata, K.
Nucleoside diphosphate glucose pyrophopshohydrolase in mast cell tumors
Agric. Biol. Chem.
37
75-81
1973
Streptomyces lavendulae, Streptomyces sp., Streptomyces fradiae, Streptomyces griseus, Streptomyces olivaceus, Streptomyces purpurascens, Streptomyces rimosus, Streptomyces ruber, Streptomyces scabiei, Streptomyces sp. AKU 2801
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Akutsu, J.; Zhang, Z.; Morita, R.; Kawarabayasi, Y.
Identification and characterization of a thermostable bifunctional enzyme with phosphomannose isomerase and sugar-1-phosphate nucleotidylyltransferase activities from a hyperthermophilic archaeon, Pyrococcus horikoshii OT3
Extremophiles
19
1077-1085
2015
Pyrococcus horikoshii (O58649), Pyrococcus horikoshii ATCC 700860 (O58649)
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