Any feedback?
No. of entries
Information on EC 2.3.1.116 - flavonol-3-O-beta-glucoside O-malonyltransferase
for references in articles please use BRENDA:EC2.3.1.116
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree 2 Transferases
2.3 Acyltransferases
2.3.1 Transferring groups other than aminoacyl groups
2.3.1.116 flavonol-3-O-beta-glucoside O-malonyltransferase
The enzyme appears in viruses and cellular organisms
- 2.3.1.116
-
mating
-
zygote
- chlamydomonas
-
meiosis
- reinhardtii
Reaction Schemes
showSynonyms
mat-3, more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
malonyl-coenzyme A:flavonol-3-O-glucoside malonyltransferase
-
-
-
-
malonyltransferase, flavonol 3-O-glucoside
-
-
-
-
MAT-3
-
-
-
-
MaT1
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
malonyl-CoA + flavonol 3-O-beta-D-glucoside = CoA + flavonol 3-O-(6-O-malonyl-beta-D-glucoside)
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Acyl group transfer
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PATHWAY SOURCE
PATHWAYS
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
malonyl-CoA:flavonol-3-O-beta-D-glucoside 6''-O-malonyltransferase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
78413-11-3
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
malonyl-CoA + genistein 7-O-glucoside
CoA + ?
-
Substrates: 7.6% of the activity with quercetin 3-O-glucoside
Products: -
Products: -
?
malonyl-CoA + isorhamnetin 3-O-glucoside
CoA + isorhamnetin 3-O-glucoside malonylester
-
Substrates: 78% of the activity with kaempferol 3-O-glucoside
Products: probably acylated at position 6 of glucose
Products: probably acylated at position 6 of glucose
?
malonyl-CoA + p-nitrophenyl beta-D-glucopyranoside
CoA + p-nitrophenyl 6-O-malonyl-beta-D-glucopyranoside
malonyl-CoA + phenyl beta-D-glucopyranoside
CoA + phenyl 6-O-malonyl-beta-D-glucopyranoside
malonyl-CoA + quercetin 3-O-glucoside
CoA + quercetin 3-O-(6-O-malonyl-beta-D-glucoside)
-
Substrates: regiospecific transfer of malonyl group to the 6-hydroxyl group of quercetin 3-O-glucoside, highly specific for these acyl donor and acceptor
Products: -
Products: -
?
malonyl-CoA + quercetin 3-O-glucoside
CoA + quercetin 3-O-(6-O-malonylbeta-D-glucoside)
-
Substrates: regiospecific transfer of malonyl group to the 6-hydroxyl group of quercetin 3-O-glucoside, highly specific for these acyl donor and acceptor
Products: -
Products: -
?
malonyl-CoA + quercetin 3-O-glucoside
CoA + quercetin 3-O-glucoside malonylester
-
Substrates: 60% of the activity with kaempferol 3-O-glucoside
Products: probably acylated at position 6 of glucose
Products: probably acylated at position 6 of glucose
?
succinyl-CoA + quercetin 3-O-glucoside
CoA + quercetin 3-(6-O-succinylbeta-D-glucoside)
-
Substrates: 27% of the activity with malonyl-CoA
Products: -
Products: -
?
succinyl-CoA + quercetin 3-O-glucoside
CoA + quercetin 3-O-(6-O-succinyl-beta-D-glucoside)
-
Substrates: 19% of the activity with malonyl-CoA
Products: -
Products: -
?
malonyl-CoA + kaempferol 3-O-glucoside
CoA + kaempferol 3-O-glucoside malonylester
-
Substrates: -
Products: -
Products: -
?
malonyl-CoA + kaempferol 3-O-glucoside
CoA + kaempferol 3-O-glucoside malonylester
-
Substrates: -
Products: probably acylated at position 6 of glucose
Products: probably acylated at position 6 of glucose
?
malonyl-CoA + p-nitrophenyl beta-D-glucopyranoside
CoA + p-nitrophenyl 6-O-malonyl-beta-D-glucopyranoside
-
Substrates: 48% of the activity with quercetin 3-O-glucoside
Products: -
Products: -
?
malonyl-CoA + p-nitrophenyl beta-D-glucopyranoside
CoA + p-nitrophenyl 6-O-malonyl-beta-D-glucopyranoside
-
Substrates: 19% of the activity with quercetin 3-O-glucoside
Products: -
Products: -
?
malonyl-CoA + phenyl beta-D-glucopyranoside
CoA + phenyl 6-O-malonyl-beta-D-glucopyranoside
-
Substrates: 6.4% of the activity with quercetin 3-O-glucoside
Products: -
Products: -
?
malonyl-CoA + phenyl beta-D-glucopyranoside
CoA + phenyl 6-O-malonyl-beta-D-glucopyranoside
-
Substrates: 11% of the activity with quercetin 3-O-glucoside
Products: -
Products: -
?
additional information
?
-
-
Substrates: less than 2% of the activity with quercetin 3-O-glucoside: pelargonidine, cyanidin, delphinidin, daidzein, o-(hydroxymethyl)phenyl beta-D-glucopyranoside, less than 2% of the activity with malonyl-CoA: acetyl-CoA, methylmalonyl-CoA
Products: -
Products: -
?
additional information
?
-
-
Substrates: less than 2% of the activity with quercetin 3-O-glucoside: pelargonidine, cyanidin, delphinidin, daidzein, genistein, o-(hydroxymethyl)phenyl beta-D-glucopyranoside, less than 2% of the activity with malonyl-CoA: acetyl-CoA, methylmalonyl-CoA
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
malonyl-CoA + kaempferol 3-O-glucoside
CoA + kaempferol 3-O-glucoside malonylester
-
Substrates: -
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). MAT1_BEMTA
461
0
51733
Swiss-Prot
Secretory Pathway (Reliability: 5)
PMAT1_ARATH
469
0
51765
Swiss-Prot
other Location (Reliability: 2)
MAT1_TOBAC
453
0
50763
Swiss-Prot
other Location (Reliability: 3)
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
50000
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C, partially purified enzyme is stable for several months, extensively purified enzyme loses 50% of activity within 2-3 days
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Matern, U.; Feser, C.; Hammer, D.
Further characterization and regulation of malonyl-coenzyme A: flavonoid glucoside malonyltransferases from parsley cell suspension cultures
Arch. Biochem. Biophys.
226
206-217
1983
Petroselinum crispum
brenda
Matern, U.; Potts, J.R.M.; Hahlbrock, K.
Two flavonoid-specific malonyltransferases from cell suspension cultures of Petroselinum hortense: partial purification and some properties of malonyl-coenzyme A: flavone/flavonol-7-O-glycoside malonyltransferase and malonyl-coenzyme A: flavonol-3-O-glucoside malonyltransferase
Arch. Biochem. Biophys.
208
233-241
1981
Petroselinum crispum
brenda
Suzuki, H.; Nakayama, T.; Nagae, S.; Yamaguchi, M.A.; Iwashita, T.; Fukui, Y.; Nishino, T.
cDNA cloning and functional characterization of flavonol 3-O-glucoside-6''-O-malonyltransferases from flowers of Verbena hybrida and Lamium purpureum
J. Mol. Catal. B
28
87-93
2004
Glandularia x hybrida, Lamium purpureum
-
brenda
EXTERNAL LINKS (specific for EC-Number 2.3.1.116)
html completed
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
About BRENDA
Social media
Help
Survey
Download
Contribution
Legal
Curated at
Member of
