Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.116 extracted from

  • Matern, U.; Potts, J.R.M.; Hahlbrock, K.
    Two flavonoid-specific malonyltransferases from cell suspension cultures of Petroselinum hortense: partial purification and some properties of malonyl-coenzyme A: flavone/flavonol-7-O-glycoside malonyltransferase and malonyl-coenzyme A: flavonol-3-O-glucoside malonyltransferase (1981), Arch. Biochem. Biophys., 208, 233-241.
    View publication on PubMed
Search for more literature for 2.3.1.116

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 kaempferol 3-O-glucoside
-
 Petroselinum crispum
0.005
-
 malonyl-CoA
-
 Petroselinum crispum
0.006
-
 isorhamnetin 3-O-glucoside
-
 Petroselinum crispum

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Petroselinum crispum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 gel filtration Petroselinum crispum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
malonyl-CoA + kaempferol 3-O-glucoside Petroselinum crispum
-
 CoA + kaempferol 3-O-glucoside malonylester
-
 ?

Organism

Organism UniProt Comment Textmining
Petroselinum crispum
-
 parsley
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Petroselinum crispum

Purification (Commentary)

Purification (Comment) Organism
-
 Petroselinum crispum

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Petroselinum crispum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Petroselinum crispum

Storage Stability

Storage Stability Organism
-70°C, partially purified enzyme is stable for several months, extensively purified enzyme loses 50% of activity within 2-3 days Petroselinum crispum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-CoA + isorhamnetin 3-O-glucoside 78% of the activity with kaempferol 3-O-glucoside Petroselinum crispum CoA + isorhamnetin 3-O-glucoside malonylester probably acylated at position 6 of glucose ?
malonyl-CoA + kaempferol 3-O-glucoside
-
 Petroselinum crispum CoA + kaempferol 3-O-glucoside malonylester
-
 ?
malonyl-CoA + kaempferol 3-O-glucoside
-
 Petroselinum crispum CoA + kaempferol 3-O-glucoside malonylester probably acylated at position 6 of glucose ?
malonyl-CoA + quercetin 3-O-glucoside 60% of the activity with kaempferol 3-O-glucoside Petroselinum crispum CoA + quercetin 3-O-glucoside malonylester probably acylated at position 6 of glucose ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 most active in Tris buffer containing bovine serum albumin Petroselinum crispum