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IUBMB Comments This two-component enzyme from Pseudomonas belongs to the family of xanthine dehydrogenases, but differs from most other members of this family. While most members contain an FAD cofactor, the large subunit of this enzyme contains three c -type cytochromes, enabling it to interact with the electron transfer chain, probably by delivering the electrons to a cytochrome o xidase. The small subunit contains a typical molybdopterin cytosine dinucleotide(MCD) cofactor and two [2Fe-2S] clusters .
Synonyms
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ndhSL
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nicotinate + a ferricytochrome + H2O = 6-hydroxynicotinate + a ferrocytochrome + 2 H+
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MetaCyc
nicotinate degradation I
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nicotinate:cytochrome 6-oxidoreductase (hydroxylating)
This two-component enzyme from Pseudomonas belongs to the family of xanthine dehydrogenases, but differs from most other members of this family. While most members contain an FAD cofactor, the large subunit of this enzyme contains three c-type cytochromes, enabling it to interact with the electron transfer chain, probably by delivering the electrons to a cytochrome oxidase. The small subunit contains a typical molybdopterin cytosine dinucleotide(MCD) cofactor and two [2Fe-2S] clusters [1].
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3-cyanopyridine + 5-methylphenazinium methyl sulfate + H2O
?
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Substrates: 4% of the activity with nicotinate
?
nicotinic acid + 5-methylphenazinium methyl sulfate + H2O
6-hydroxynicotinate + ?
nicotinic acid + 5-methylphenazinium methyl sulfate + H2O
6-hydroxynicotinic acid + ?
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Substrates: -
?
nipecotic acid + 5-methylphenazinium methyl sulfate + H2O
?
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Substrates: 8% of the activity with nicotinate
?
picolinic acid + 5-methylphenazinium methyl sulfate + H2O
?
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Substrates: 7% of the activity with nicotinate
?
pyrazine-2-carboxylic acid + 5-methylphenazinium methyl sulfate + H2O
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Substrates: 8% of the activity with nicotinate
?
pyridine-3-carboxyaldehyde + 5-methylphenazinium methyl sulfate + H2O
?
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Substrates: 5% of the activity with nicotinate
?
pyridine-3-sulfonic acid + 5-methylphenazinium methyl sulfate + H2O
?
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Substrates: 4% of the activity with nicotinate
?
nicotinic acid + 5-methylphenazinium methyl sulfate + H2O
6-hydroxynicotinate + ?
Substrates: -
?
nicotinic acid + 5-methylphenazinium methyl sulfate + H2O
6-hydroxynicotinate + ?
Substrates: -
?
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molybdopterin cytosine dinucleotide
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2Fe-2S-center
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cytochrome c
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Molybdenum
subunit NdhL contains a molybdenum cofactor
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KT2440
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brenda
subunit ndhL; KT2440
SwissProt
brenda
subunit ndhS; KT2440
UniProt
brenda
KT2440
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brenda
subunit ndhL; KT2440
SwissProt
brenda
subunit ndhS; KT2440
UniProt
brenda
Highest Expressing Human Cell Lines
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Cell Line Links
Gene Links
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NICB_PSEPK
Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
1187
0
127875
Swiss-Prot
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NICA_PSEPK
Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
157
0
16686
Swiss-Prot
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127874
x * 127874, subunit NdhSL, calculated. x * 130000, product of gene cluster ndhSL, SDS-PAGE
12800
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x * 23800, small subunit NicA, + x * 12800, large subunit NicB
16685
x * 16685, subunit NdhS, calculated. x * 130000, product of gene cluster ndhSL, SDS-PAGE
23800
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x * 23800, small subunit NicA, + x * 12800, large subunit NicB
130000
x * 127874, subunit NdhSL, calculated. x * 130000, product of gene cluster ndhSL, SDS-PAGE
130000
x * 16685, subunit NdhS, calculated. x * 130000, product of gene cluster ndhSL, SDS-PAGE
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?
x * 16685, subunit NdhS, calculated. x * 130000, product of gene cluster ndhSL, SDS-PAGE
?
x * 127874, subunit NdhSL, calculated. x * 130000, product of gene cluster ndhSL, SDS-PAGE
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x * 23800, small subunit NicA, + x * 12800, large subunit NicB
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x * 127874, subunit NdhSL, calculated. x * 130000, product of gene cluster ndhSL, SDS-PAGE
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?
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x * 16685, subunit NdhS, calculated. x * 130000, product of gene cluster ndhSL, SDS-PAGE
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additional information
subunit NdhS contains a 2Fe-2S iron-sulfur domain
additional information
subunit NdhS contains a 2Fe-2S iron-sulfur domain
additional information
subunit NdhL contains a molybdenum cofactor and cytochrome c
additional information
subunit NdhL contains a molybdenum cofactor and cytochrome c
additional information
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small subunit NicA harbors the 41-C-X4-C-G-X-C-Xn-C-59 and 100-C-G-X-C-X31-C-X-C-137 conserved motifs likely involved in binding of the two [2Fe-2S] clusters
additional information
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the N-terminal region of large subunit NicB contains the active site and the molybdopterin cytosine dinucleotide binding sites in an arrangement MPT2-MPT1-MPT3. The C-terminal region of NicB, residues750-1187, contains three conserved cytochrome c hemebinding motifs 817-CAVCH-823, 963-CTACH-969, and 1087-CLGCH-1093
additional information
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subunit NdhL contains a molybdenum cofactor and cytochrome c
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additional information
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subunit NdhS contains a 2Fe-2S iron-sulfur domain
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expression of gene cluster ndhSL in Pseudomonas entomophila
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Yang, Y.; Yuan, S.; Chen, T.; Ma, P.; Shang, G.; Dai, Y.
Cloning, heterologous expression, and functional characterization of the nicotinate dehydrogenase gene from Pseudomonas putida KT2440
Biodegradation
20
541-549
2009
Pseudomonas putida (Q88FX8), Pseudomonas putida (Q88FX9), Pseudomonas putida KT 2240 (Q88FX8), Pseudomonas putida KT 2240 (Q88FX9)
brenda
Jimenez, J.; Canales, A.; Jimenez-Barbero, J.; Ginalski, K.; Rychlewski, L.; Garcia, J.; Diaz, E.
Deciphering the genetic determinants for aerobic nicotinic acid degradation: The nic cluster from Pseudomonas putida KT2440
Proc. Natl. Acad. Sci. USA
105
11329-11334
2008
Pseudomonas putida, Pseudomonas putida KT 2240
brenda
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