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testosterone,reduced-ferredoxin:oxygen oxidoreductase (1alpha-hydroxylating)
The enzyme, characterized from the bacterium Sorangium cellulosum, is a class I cytochrome P-450, and uses ferredoxin as its electron donor [1]. It was shown to act on several C-19 steroid substrates, including testosterone, androstenedione, testosterone-acetate and 11-oxoandrostenedione [2].
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(+)-nootkatone + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
?
11-oxoandrostenedione + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
1alpha-hydroxy-11-oxoandrostenedione + H2O + 2 oxidized ferredoxin [iron-sulfur] cluster
Substrates: Escherichia coli-based whole-cell biocatalyst system
Products: -
?
androstenedione + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
1alpha-hydroxyandrostenedione + H2O + 2 oxidized ferredoxin [iron-sulfur] cluster
Substrates: Escherichia coli-based whole-cell biocatalyst system
Products: -
?
androstenedione + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
?
testosterone + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
1alpha-hydroxytestosterone + H2O + 2 oxidized ferredoxin [iron-sulfur] cluster
testosterone + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
1alpha-hydroxytestosterone + H2O + oxidized ferredoxin [iron-sulfur] cluster
zerumbone + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
?
(+)-nootkatone + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
?
Substrates: -
Products: -
?
(+)-nootkatone + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
?
Substrates: -
Products: -
?
androstenedione + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
?
Substrates: -
Products: -
?
androstenedione + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
?
Substrates: -
Products: -
?
testosterone + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
1alpha-hydroxytestosterone + H2O + 2 oxidized ferredoxin [iron-sulfur] cluster
-
Substrates: -
Products: -
?
testosterone + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
1alpha-hydroxytestosterone + H2O + 2 oxidized ferredoxin [iron-sulfur] cluster
Substrates: Escherichia coli-based whole-cell biocatalyst system
Products: -
?
testosterone + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
1alpha-hydroxytestosterone + H2O + 2 oxidized ferredoxin [iron-sulfur] cluster
Substrates: Escherichia coli-based whole-cell biocatalyst system
Products: Escherichia coli-based whole-cell biocatalyst system produces a mixture (1:2.5:3) of an unknown compound and 1alpha- and 9alpha-hydroxytestosterone acetate
?
testosterone + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
1alpha-hydroxytestosterone + H2O + oxidized ferredoxin [iron-sulfur] cluster
Substrates: -
Products: -
?
testosterone + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
1alpha-hydroxytestosterone + H2O + oxidized ferredoxin [iron-sulfur] cluster
Substrates: -
Products: -
?
zerumbone + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
?
Substrates: -
Products: -
?
zerumbone + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
?
Substrates: -
Products: -
?
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testosterone + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
1alpha-hydroxytestosterone + H2O + oxidized ferredoxin [iron-sulfur] cluster
testosterone + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
1alpha-hydroxytestosterone + H2O + oxidized ferredoxin [iron-sulfur] cluster
Substrates: -
Products: -
?
testosterone + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
1alpha-hydroxytestosterone + H2O + oxidized ferredoxin [iron-sulfur] cluster
Substrates: -
Products: -
?
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Ewen, K.M.; Hannemann, F.; Khatri, Y.; Perlova, O.; Kappl, R.; Krug, D.; Huttermann, J.; Muller, R.; Bernhardt, R.
Genome mining in Sorangium cellulosum So ce56: identification and characterization of the homologous electron transfer proteins of a myxobacterial cytochrome P450
J. Biol. Chem.
284
28590-28598
2009
Sorangium cellulosum
brenda
Kuzikov, A.V.; Masamrekh, R.A.; Khatri, Y.; Zavialova, M.G.; Bernhardt, R.; Archakov, A.I.; Shumyantseva, V.V.
Scrutiny of electrochemically-driven electrocatalysis of C-19 steroid 1alpha-hydroxylase (CYP260A1) from Sorangium cellulosum So ce56
Anal. Biochem.
513
28-35
2016
Sorangium cellulosum (A9FDB7), Sorangium cellulosum So ce56 (A9FDB7), Sorangium cellulosum So ce56
brenda
Schifrin, A.; Litzenburger, M.; Ringle, M.; Ly, T.T.; Bernhardt, R.
New sesquiterpene oxidations with CYP260A1 and CYP264B1 from Sorangium cellulosum So ce56
ChemBioChem
16
2624-2632
2015
Sorangium cellulosum (A9FDB7), Sorangium cellulosum, Sorangium cellulosum So ce56 (A9FDB7)
brenda
Khatri, Y.; Ringle, M.; Lisurek, M.; von Kries, J.P.; Zapp, J.; Bernhardt, R.
Substrate hunting for the myxobacterial CYP260A1 revealed new 1alpha-hydroxylated products from C-19 steroids
Chembiochem
17
90-101
2016
Sorangium cellulosum (A9FDB7), Sorangium cellulosum
brenda
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