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IUBMB Comments A cytochrome P-450 (heme-thiolate) protein. The enzyme from rice (Oryza sativa) is involved in the biosynthesis of the antifungal phytocassanes.
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
cyp76m7,
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ent-cassadiene C11alpha-hydroxylase
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CYP76M7
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ent-cassa-12,15-diene + O2 + [reduced NADPH-hemoprotein reductase] = ent-11beta-hydroxycassa-12,15-diene + [oxidized NADPH-hemoprotein reductase] + H2O
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ent-cassa-12,15-diene,[reduced NADPH-hemoprotein reductase]:oxygen 11-oxidoreductase
A cytochrome P-450 (heme-thiolate) protein. The enzyme from rice (Oryza sativa) is involved in the biosynthesis of the antifungal phytocassanes.
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ent-cassa-12,15-diene + [reduced NADPH-hemoprotein reductase] + O2
11-(S)-hydroxy-entcassa-12,15-diene + [oxidized NADPH-hemoprotein reductase] + H2O
specific substrate, C11alpha hydroxylation
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additional information
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additional information
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only trace amounts of hydroxylation (less than 1% conversion) is observed for ent-pimaradiene, ent-sandaracopimaradiene, ent-kaurene, ent-isokaurene, synpimaradiene, syn-stemarene, or syn-stemodene
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additional information
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only trace amounts of hydroxylation (less than 1% conversion) is observed for ent-pimaradiene, ent-sandaracopimaradiene, ent-kaurene, ent-isokaurene, synpimaradiene, syn-stemarene, or syn-stemodene
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0.039
ent-cassa-12,15-diene
in 0.1 M Tris-HCl, pH 7.2, 20% (v/v) glycerol, 0.5 mM EDTA, 1 mM dithiothreitol, temperature not specified in the publication
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0.13
using ent-cassa-12,15-diene as substrate in 0.1 M Tris-HCl, pH 7.2, 20% (v/v) glycerol, 0.5 mM EDTA, 1 mM dithiothreitol, temperature not specified in the publication
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brenda
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UniProt
brenda
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malfunction
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RNAi-mediated double knockdown of CYP76M7 and -8 suppresses elicitor inducible phytocassane production, indicating a role for these monooxygenases in phytocassane biosynthesis
physiological function
the enzyme acts in the production of antifungal phytocassanes
physiological function
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the enzyme mediates an early step in biosynthesis of the derived phytocassane class of phytoalexins
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C76M7_ORYSJ
500
1
55522
Swiss-Prot
Mitochondrion (Reliability: 3 )
C76M8_ORYSJ
500
1
55933
Swiss-Prot
Mitochondrion (Reliability: 3 )
A0A084GEV4_PSEDA
544
0
61784
TrEMBL
Secretory Pathway (Reliability: 4 )
A0A084G5A2_PSEDA
144
0
16098
TrEMBL
other Location (Reliability: 1 )
Q6YTE2_ORYSJ
499
0
55419
TrEMBL
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expressed in Escherichia coli strain C41 and in Sf21 insect cells
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Wang, Q.; Hillwig, M.; Okada, K.; Yamazaki, K.; Wu, Y.; Swaminathan, S.; Yamane, H.; Peters, R.
Characterization of CYP76M5-8 indicates metabolic plasticity within a plant biosynthetic gene cluster
J. Biol. Chem.
287
6159-6168
2012
Oryza sativa
brenda
Swaminathan, S.; Morrone, D.; Wang, Q.; Bruce Fulton, D.; Peters, R.
CYP76M7 is an ent-cassadiene C11 alpha-hydroxylase defining a second multifunctional diterpenoid biosynthetic gene cluster in rice
Plant Cell
21
3315-3325
2009
Oryza sativa (Q6YTE2), Oryza sativa
brenda
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1.14.14.112 ent-cassa-12,15-diene 11-hydroxylase
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