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IUBMB Comments A cytochrome P -450 (heme-thiolate) protein. The enzyme from rice (Oryza sativa ) is involved in the biosynthesis of the antifungal phytocassanes.
Synonyms
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EC 1.14.13.145
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formerly
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ent-cassadiene C11alpha-hydroxylase
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CYP76M7
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ent-cassa-12,15-diene + O2 + [reduced NADPH-hemoprotein reductase] = ent-11beta-hydroxycassa-12,15-diene + [oxidized NADPH-hemoprotein reductase] + H2O
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MetaCyc
phytocassanes biosynthesis, shared reactions
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ent-cassa-12,15-diene,[reduced NADPH-hemoprotein reductase]:oxygen 11-oxidoreductase
A cytochrome P-450 (heme-thiolate) protein. The enzyme from rice (Oryza sativa) is involved in the biosynthesis of the antifungal phytocassanes.
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ent-cassa-12,15-diene + [reduced NADPH-hemoprotein reductase] + O2
11-(S)-hydroxy-entcassa-12,15-diene + [oxidized NADPH-hemoprotein reductase] + H2O
Substrates: specific substrate, C11alpha hydroxylation
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additional information
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additional information
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Substrates: only trace amounts of hydroxylation (less than 1% conversion) is observed for ent-pimaradiene, ent-sandaracopimaradiene, ent-kaurene, ent-isokaurene, synpimaradiene, syn-stemarene, or syn-stemodene
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additional information
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Substrates: only trace amounts of hydroxylation (less than 1% conversion) is observed for ent-pimaradiene, ent-sandaracopimaradiene, ent-kaurene, ent-isokaurene, synpimaradiene, syn-stemarene, or syn-stemodene
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0.039
ent-cassa-12,15-diene
in 0.1 M Tris-HCl, pH 7.2, 20% (v/v) glycerol, 0.5 mM EDTA, 1 mM dithiothreitol, temperature not specified in the publication
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0.13
using ent-cassa-12,15-diene as substrate in 0.1 M Tris-HCl, pH 7.2, 20% (v/v) glycerol, 0.5 mM EDTA, 1 mM dithiothreitol, temperature not specified in the publication
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SwissProt
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UniProt
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Highest Expressing Human Cell Lines
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Cell Line Links
Gene Links
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malfunction
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RNAi-mediated double knockdown of CYP76M7 and -8 suppresses elicitor inducible phytocassane production, indicating a role for these monooxygenases in phytocassane biosynthesis
malfunction
the cyp76m7/8 double mutant exhibits significant reductions of both phytocassanes or momilactones, with no consistent effect on either oryzalexin S or oryzalexins A-F
physiological function
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the enzyme mediates an early step in biosynthesis of the derived phytocassane class of phytoalexins
physiological function
the enzyme acts in the production of antifungal phytocassanes
physiological function
the enzyme is associated with the production of phytocassanes
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C76M7_ORYSJ
500
1
55522
Swiss-Prot
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C76M8_ORYSJ
500
1
55933
Swiss-Prot
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Q6YTE2_ORYSJ
499
0
55419
TrEMBL
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expressed in Escherichia coli strain C41 and in Sf21 insect cells
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Wang, Q.; Hillwig, M.; Okada, K.; Yamazaki, K.; Wu, Y.; Swaminathan, S.; Yamane, H.; Peters, R.
Characterization of CYP76M5-8 indicates metabolic plasticity within a plant biosynthetic gene cluster
J. Biol. Chem.
287
6159-6168
2012
Oryza sativa
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Swaminathan, S.; Morrone, D.; Wang, Q.; Bruce Fulton, D.; Peters, R.
CYP76M7 is an ent-cassadiene C11 alpha-hydroxylase defining a second multifunctional diterpenoid biosynthetic gene cluster in rice
Plant Cell
21
3315-3325
2009
Oryza sativa (Q6YTE2), Oryza sativa
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Li, R.; Zhang, J.; Li, Z.; Peters, R.J.; Yang, B.
Dissecting the labdane-related diterpenoid biosynthetic gene clusters in rice reveals directional cross-cluster phytotoxicity
New Phytol.
233
878-889
2022
Oryza sativa Japonica Group (Q69X58)
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