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Literature summary for 1.14.14.112 extracted from

  • Swaminathan, S.; Morrone, D.; Wang, Q.; Bruce Fulton, D.; Peters, R.
    CYP76M7 is an ent-cassadiene C11 alpha-hydroxylase defining a second multifunctional diterpenoid biosynthetic gene cluster in rice (2009), Plant Cell, 21, 3315-3325.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain C41 and in Sf21 insect cells Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.039
-
ent-cassa-12,15-diene in 0.1 M Tris-HCl, pH 7.2, 20% (v/v) glycerol, 0.5 mM EDTA, 1 mM dithiothreitol, temperature not specified in the publication Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa Q6YTE2
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.13
-
using ent-cassa-12,15-diene as substrate in 0.1 M Tris-HCl, pH 7.2, 20% (v/v) glycerol, 0.5 mM EDTA, 1 mM dithiothreitol, temperature not specified in the publication Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ent-cassa-12,15-diene + [reduced NADPH-hemoprotein reductase] + O2 specific substrate, C11alpha hydroxylation Oryza sativa 11-(S)-hydroxy-entcassa-12,15-diene + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
additional information only trace amounts of hydroxylation (less than 1% conversion) is observed for ent-pimaradiene, ent-sandaracopimaradiene, ent-kaurene, ent-isokaurene, synpimaradiene, syn-stemarene, or syn-stemodene Oryza sativa ?
-
?

Synonyms

Synonyms Comment Organism
CYP76M7
-
Oryza sativa
ent-cassadiene C11alpha-hydroxylase
-
Oryza sativa

General Information

General Information Comment Organism
physiological function the enzyme acts in the production of antifungal phytocassanes Oryza sativa