Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain C41 and in Sf21 insect cells | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.039 | - |
ent-cassa-12,15-diene | in 0.1 M Tris-HCl, pH 7.2, 20% (v/v) glycerol, 0.5 mM EDTA, 1 mM dithiothreitol, temperature not specified in the publication | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | Q6YTE2 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.13 | - |
using ent-cassa-12,15-diene as substrate in 0.1 M Tris-HCl, pH 7.2, 20% (v/v) glycerol, 0.5 mM EDTA, 1 mM dithiothreitol, temperature not specified in the publication | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ent-cassa-12,15-diene + [reduced NADPH-hemoprotein reductase] + O2 | specific substrate, C11alpha hydroxylation | Oryza sativa | 11-(S)-hydroxy-entcassa-12,15-diene + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
additional information | only trace amounts of hydroxylation (less than 1% conversion) is observed for ent-pimaradiene, ent-sandaracopimaradiene, ent-kaurene, ent-isokaurene, synpimaradiene, syn-stemarene, or syn-stemodene | Oryza sativa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP76M7 | - |
Oryza sativa |
ent-cassadiene C11alpha-hydroxylase | - |
Oryza sativa |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme acts in the production of antifungal phytocassanes | Oryza sativa |