Information on EC 1.1.1.80 - isopropanol dehydrogenase (NADP+)

for references in articles please use BRENDA:EC1.1.1.80
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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

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EC NUMBER
COMMENTARY hide
1.1.1.80
-
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RECOMMENDED NAME
GeneOntology No.
isopropanol dehydrogenase (NADP+)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
propan-2-ol + NADP+ = acetone + NADPH + H+
show the reaction diagram
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-
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
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redox reaction
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-
-
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reduction
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-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
propanol degradation
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Propanoate metabolism
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acetone degradation I (to methylglyoxal)
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isopropanol biosynthesis (engineered)
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acetone degradation II (to acetoacetate)
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acetone degradation III (to propane-1,2-diol)
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SYSTEMATIC NAME
IUBMB Comments
propan-2-ol:NADP+ oxidoreductase
Also acts on other short-chain secondary alcohols and, slowly, on primary alcohols.
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CAS REGISTRY NUMBER
COMMENTARY hide
37250-18-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
putative
UniProt
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-methyl-1-pentanol + NAD+
4-methyl-1-pentanal + NADH
show the reaction diagram
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-
-
-
?
4-methyl-1-pentanol + NADP+
4-methyl-1-pentanal + NADPH
show the reaction diagram
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-
-
-
?
6-methyl-2-heptanol + NAD+
6-methyl-2-heptanone + NADH + H+
show the reaction diagram
-
-
-
-
?
6-methyl-2-heptanol + NADP+
6-methyl-2-heptanone + NADPH
show the reaction diagram
-
-
-
-
?
acetaldehyde + NADPH + H+
ethanol + NADP+
show the reaction diagram
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-
-
r
acetone + NADPH + H+
propan-2-ol + NADP+
show the reaction diagram
butan-2-ol + NADP+
butanone + NADPH + H+
show the reaction diagram
preferred direction of the reaction is reduction rather than oxidation
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-
r
butanone + NADPH + H+
butan-2-ol + NADP+
show the reaction diagram
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-
-
r
ethanol + NAD+
ethanal + NADH
show the reaction diagram
-
-
-
-
?
ethanol + NADP+
acetaldehyde + NADPH + H+
show the reaction diagram
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-
-
r
ethanol + NADP+
ethanal + NADPH + H+
show the reaction diagram
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-
-
-
?
pentan-2-ol + NADP+
pentan-2-one + NADPH + H+
show the reaction diagram
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-
-
r
pentan-3-ol + NADP+
pentan-3-one + NADPH + H+
show the reaction diagram
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-
-
r
pentan-3-one + NADPH + H+
pentan-3-ol + NADP+
show the reaction diagram
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r
propan-1-ol + NADP+
propanal + NADPH + H+
show the reaction diagram
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-
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r
propan-2-ol + NAD+
acetone + NADH
show the reaction diagram
-
NAD+ effectively substitutes for NADP+
-
?
propan-2-ol + NADP+
acetone + NADPH
show the reaction diagram
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-
-
?
propan-2-ol + NADP+
acetone + NADPH + H+
show the reaction diagram
additional information
?
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
butan-2-ol + NADP+
butanone + NADPH + H+
show the reaction diagram
A2DJ40
preferred direction of the reaction is reduction rather than oxidation
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-
r
propan-2-ol + NADP+
acetone + NADPH + H+
show the reaction diagram
A2DJ40
preferred direction of the reaction is reduction rather than oxidation
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-
r
additional information
?
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possibly takes part in the further metabolic reactions of the fragments formed from the side-chain of the steroid hormone precursor in the adrenals or gonads
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NAD+
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effectively substitutes for NADP+
NADP+
NADPH
additional information
no cofactor: NADH
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,2'-dipyridyl
5 mM, 80% residual activity
4-hydroxymercuribenzoic acid
0.1 mM, 49% residual activity
4-Methylpyrazole
5 mM, 68% residual activity
iodacetamide
50 mM, 49% residual activity
pyrazole
5 mM, 74% residual activity
additional information
not inhibitory: EDTA up to 5 mM
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00071
4-Methyl-1-pentanol
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-
0.00259
6-methyl-2-heptanol
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0.00028
acetaldehyde
37°C, pH 7.0
0.000093 - 0.6
acetone
0.00078
butan-2-ol
37°C, pH 9.0
0.000061
Butanone
37°C, pH 7.0
0.00024 - 0.1
ethanol
0.0052
NADP+
pH 7.0, 37°C, cosubstrate acetone
0.51
NADPH
pH 9.0, 37°C, cosubstrate propan-2-ol
0.0056
pentan-2-ol
37°C, pH 9.0
0.0015
pentan-3-ol
37°C, pH 9.0
0.000256
pentan-3-one
37°C, pH 7.0
0.0275
propan-1-ol
37°C, pH 9.0
0.00056 - 0.00178
propan-2-ol
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5
reduction of acetone, citrate buffer
6.4 - 7
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in mitochondria
6.5 - 7.5
reduction of acetone, Bis/Tris propane buffer
8 - 8.5
oxidation of propan-2-ol, Bis/Tris propane buffer
8.2
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in cytoplasma
9.5
oxidation of propan-2-ol, glycine/HCl buffer
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
activity detected in mitochondria and cytoplasm
Manually annotated by BRENDA team
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
39100
2 * 43000, SDS-PAGE, 2 * 39100, calculated
43000
2 * 43000, SDS-PAGE, 2 * 39100, calculated
81000
gel filtration
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 43000, SDS-PAGE, 2 * 39100, calculated
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
iron limitation results in a 1.5fold increase in enzyme mRNA
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
diagnostics
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characterization of adrenal and gonadal tissue
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LINKS TO OTHER DATABASES (specific for EC-Number 1.1.1.80)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)