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Literature summary for 1.1.1.80 extracted from

  • Jiang, W.; Fang, B.S.
    Coenzyme binding site analysis of an isopropanol dehydrogenase with wide substrate spectrum and excellent organic solvent tolerance (2020), Appl. Biochem. Biotechnol., 190, 18-29 .
    View publication on PubMed
Search for more literature for 1.1.1.80

Application

Application Comment Organism
additional information the enzyme is an ideal candidate biocatalyst in the construction of coenzyme regeneration system and the enzymatic bioconversion of high value alcohols or other compounds Aspergillus fumigatus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Aspergillus fumigatus

General Stability

General Stability Organism
unstable when isopropanol is used as the substrate Aspergillus fumigatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 propan-2-ol pH 7.0, temperature not specified in the publication Aspergillus fumigatus
0.06
-
 n-butanol pH 7.0, temperature not specified in the publication Aspergillus fumigatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40800
-
 SDS-PAGE, isopropanol dehydrogenase Aspergillus fumigatus

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone in the presence of isopropanol, acetonitrile, and acetone at 10% (v/v) or 30% (v/v), the enzyme exhibits over 45% of its maximum activity Aspergillus fumigatus
acetonitrile in the presence of isopropanol, acetonitrile, and acetone at 10% (v/v) or 30% (v/v), the enzyme exhibits over 45% of its maximum activity Aspergillus fumigatus
acetophenone strong stability in the presence of acetophenone at concentrations of 10% (v/v) Aspergillus fumigatus
Ethanol the enzyme remains less than 20% of its maximum activity in the presence of ethanol Aspergillus fumigatus
isopropanol in the presence of isopropanol, acetonitrile, and acetone at 10% (v/v) or 30% (v/v), the enzyme exhibits over 45% of its maximum activity Aspergillus fumigatus
Methanol the enzyme remains less than 20% of its maximum activity in the presence of methanol Aspergillus fumigatus

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus
-
-
-
Aspergillus fumigatus Af293
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isobutanol + NAD+
-
 Aspergillus fumigatus ? + NADH + H+
-
 ?
isobutanol + NAD+
-
 Aspergillus fumigatus Af293 ? + NADH + H+
-
 ?
isobutanol + NADP+
-
 Aspergillus fumigatus ? + NADPH + H+
-
 ?
isobutanol + NADP+
-
 Aspergillus fumigatus Af293 ? + NADPH + H+
-
 ?
n-butanol + NAD+
-
 Aspergillus fumigatus butanone + NADH + H+
-
 ?
n-butanol + NAD+
-
 Aspergillus fumigatus Af293 butanone + NADH + H+
-
 ?
n-butanol + NADP+
-
 Aspergillus fumigatus butanone + NADPH + H+
-
 ?
propan-2-ol + NAD+
-
 Aspergillus fumigatus acetone + NADH + H+
-
 ?
propan-2-ol + NAD+
-
 Aspergillus fumigatus Af293 acetone + NADH + H+
-
 ?
propan-2-ol + NADP+
-
 Aspergillus fumigatus acetone + NADPH + H+
-
 ?
propan-2-ol + NADP+
-
 Aspergillus fumigatus Af293 acetone + NADPH + H+
-
 ?

Synonyms

Synonyms Comment Organism
IDH
-
 Aspergillus fumigatus
isopropanol dehydrogenase
-
 Aspergillus fumigatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 substrate: n-butanol Aspergillus fumigatus
55
-
 substrate: isopropanol Aspergillus fumigatus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 50 35°C: about 70% of maximal activity, 50°C: about 60% of maximal activity, substrate: n-butanol Aspergillus fumigatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 45 incubation for 1 h under pH 6.0, the enzyme shows over 43% of the maximal activity between 25°C and 30°C Aspergillus fumigatus
40 45 incubation for 1 h under pH 6.0, the enzyme shows less than 10% of the maximal activity between 25°C and 30°C Aspergillus fumigatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.066
-
 propan-2-ol pH 7.0, temperature not specified in the publication Aspergillus fumigatus
0.22
-
 n-butanol pH 7.0, temperature not specified in the publication Aspergillus fumigatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 substrate: n-butanol Aspergillus fumigatus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.6
-
 without any stabilizer, the enzyme at pH 5.6 and 7.0 retains over 25% of its maximum activity after pre-treated in different buffers ranging from pH 5.6-10.0 for 27 h at 4°C Aspergillus fumigatus
7
-
 without any stabilizer, the enzyme at pH 5.6 and 7.0 retains over 25% of its maximum activity after pre-treated in different buffers ranging from pH 5.6-10.0 for 27 h at 4°C Aspergillus fumigatus

General Information

General Information Comment Organism
evolution an evolutionary tree is constructed to analyze the evolution of the enzyme illustrates that the isopropanol dehydrogenase from Aspergillus fumigatus Af293 is far from to other dehydrogenases Aspergillus fumigatus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.36
-
 propan-2-ol pH 7.0, temperature not specified in the publication Aspergillus fumigatus
3.96
-
 n-butanol pH 7.0, temperature not specified in the publication Aspergillus fumigatus