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0.00016 - 6
5-phospho-alpha-D-ribose 1-diphosphate
0.014 - 13.3
anthranilate
additional information
5-phospho-alpha-D-ribose 1-diphosphate
0.00016
5-phospho-alpha-D-ribose 1-diphosphate
-
at pH 7.5 and 25°C
0.00067
5-phospho-alpha-D-ribose 1-diphosphate
-
at pH 7.5 and 25°C
0.014
5-phospho-alpha-D-ribose 1-diphosphate
25°C
0.035
5-phospho-alpha-D-ribose 1-diphosphate
40°C
0.046
5-phospho-alpha-D-ribose 1-diphosphate
mutant enzyme I36E/M47D/T77I/D83G/F149S/I169T/F193S/L320M, pH 7.2, 37°C
0.067
5-phospho-alpha-D-ribose 1-diphosphate
50°C
0.105
5-phospho-alpha-D-ribose 1-diphosphate
60°C
0.13
5-phospho-alpha-D-ribose 1-diphosphate
-
at pH 7.5 and 25°C
0.18
5-phospho-alpha-D-ribose 1-diphosphate
at pH 7.5 and 25°C
0.25
5-phospho-alpha-D-ribose 1-diphosphate
-
at pH 7.5 and 25°C
0.27
5-phospho-alpha-D-ribose 1-diphosphate
-
at pH 7.5 and 25°C
0.28
5-phospho-alpha-D-ribose 1-diphosphate
-
at pH 7.5 and 25°C
0.4
5-phospho-alpha-D-ribose 1-diphosphate
87°C
0.42
5-phospho-alpha-D-ribose 1-diphosphate
-
at pH 7.5 and 25°C
0.43
5-phospho-alpha-D-ribose 1-diphosphate
-
at pH 7.5 and 25°C
0.44
5-phospho-alpha-D-ribose 1-diphosphate
-
at pH 7.5 and 25°C
0.48
5-phospho-alpha-D-ribose 1-diphosphate
60°C, pH 7.5, 2 mM Mg2+, mutant enzyme K106Q
0.62
5-phospho-alpha-D-ribose 1-diphosphate
60°C, pH 7.5, 0.05 mM Mg2+, mutant enzyme R164A
0.73
5-phospho-alpha-D-ribose 1-diphosphate
60°C, pH 7.5, 0.05 mM Mg2+, mutant enzyme R164A/H154A
0.78
5-phospho-alpha-D-ribose 1-diphosphate
mutant enzyme I36E/M47D/D83G/N109S/F149S, pH 7.2, 37°C
1.1
5-phospho-alpha-D-ribose 1-diphosphate
60°C, pH 7.5, 2 mM Mg2+, wild-type enzyme
1.2
5-phospho-alpha-D-ribose 1-diphosphate
mutant enzyme I36E/M47D/T77I/D83G/F149S/N109S/I169T/F193S/L320M, pH 7.2, 37°C
1.3
5-phospho-alpha-D-ribose 1-diphosphate
mutant enzyme I36E/M47D/T77I/D83G/F149S, pH 7.2, 37°C
2
5-phospho-alpha-D-ribose 1-diphosphate
60°C, pH 7.5, 0.05 mM Mg2+, mutant enzyme H107A/P178A
2.7
5-phospho-alpha-D-ribose 1-diphosphate
mutant enzyme I36E/M47D/D83G/F149S/F193S, pH 7.2, 37°C
3.4
5-phospho-alpha-D-ribose 1-diphosphate
mutant enzyme I36E/M47D/D83G/F149S, pH 7.2, 37°C
3.5
5-phospho-alpha-D-ribose 1-diphosphate
mutant enzyme I36E/M47D/D83G/F149S/I169T, pH 7.2, 37°C
3.9
5-phospho-alpha-D-ribose 1-diphosphate
mutant enzyme I36E/M47D/D83G/F149S/L320M, pH 7.2, 37°C
4.2
5-phospho-alpha-D-ribose 1-diphosphate
60°C, pH 7.5, 0.05 mM Mg2+, wild-type enzyme
4.4
5-phospho-alpha-D-ribose 1-diphosphate
-
-
5.1
5-phospho-alpha-D-ribose 1-diphosphate
60°C, pH 7.5, 0.05 mM Mg2+, mutant enzyme H107A
5.6
5-phospho-alpha-D-ribose 1-diphosphate
60°C, pH 7.5, 2 mM Mg2+, mutant enzyme E224Q
6
5-phospho-alpha-D-ribose 1-diphosphate
60°C, pH 7.5, 2 mM Mg2+, mutant enzyme D223N
0.014
anthranilate
25°C
0.014
anthranilate
at 25°C
0.035
anthranilate
at 40°C
0.046
anthranilate
mutant enzyme I36E/M47D/T77I/D83G/F149S/I169T/F193S/L320M, pH 7.2, 37°C
0.067
anthranilate
at 50°C
0.105
anthranilate
at 60°C
0.24
anthranilate
wild-type, in the presence of 2 mM MgCl2, 50 mM Tris-HCl, pH 7.2, at 37°C
0.28
anthranilate
+/-0.06, I36E/M47D double mutant, pH 7.2, 37°C, 0.01 microM subunit concentration
0.33
anthranilate
wild-type, in the presence of 0.05 mM MgCl2, 50 mM Tris-HCl, pH 7.2, at 37°C
0.34
anthranilate
-
cosubstrate: 5-phospho-alpha-D-ribose 1-diphosphate
0.41
anthranilate
+/-0.04, wild-type, pH 7.2, 37°C, 0.01 microM subunit concentration
0.48
anthranilate
60°C, pH 7.5, 2 mM Mg2+, mutant enzyme K106Q
0.62
anthranilate
60°C, pH 7.5, 0.05 mM Mg2+, mutant enzyme R164A
0.73
anthranilate
60°C, pH 7.5, 0.05 mM Mg2+,mutant enzyme R164A/H154A
0.78
anthranilate
mutant enzyme I36E/M47D/D83G/N109S/F149S, pH 7.2, 37°C
1.1
anthranilate
60°C, pH 7.5, 2 mM Mg2+, wild-type enzyme
1.2
anthranilate
mutant F149S, in the presence of 0.05 mM MgCl2, 50 mM Tris-HCl, pH 7.2, at 37°C
1.2
anthranilate
mutant enzyme I36E/M47D/T77I/D83G/F149S/N109S/I169T/F193S/L320M, pH 7.2, 37°C
1.3
anthranilate
mutant enzyme I36E/M47D/T77I/D83G/F149S, pH 7.2, 37°C
2
anthranilate
60°C, pH 7.5, 0.05 mM Mg2+, mutant enzyme H107A/P178A
2
anthranilate
mutant D83G, in the presence of 2 mM MgCl2, 50 mM Tris-HCl, pH 7.2, at 37°C
2.7
anthranilate
mutant enzyme I36E/M47D/D83G/F149S/F193S, pH 7.2, 37°C
2.9
anthranilate
-
cosubstrate: 5-phospho-alpha-D-ribose 1-diphosphate
3.4
anthranilate
mutant enzyme I36E/M47D/D83G/F149S, pH 7.2, 37°C
3.5
anthranilate
mutant enzyme I36E/M47D/D83G/F149S/I169T, pH 7.2, 37°C
3.9
anthranilate
mutant enzyme I36E/M47D/D83G/F149S/L320M, pH 7.2, 37°C
4.1
anthranilate
-
cosubstrate: 5-phospho-alpha-D-ribose 1-diphosphate
4.2
anthranilate
60°C, pH 7.5, 0.05 mM Mg2+, wild-type enzyme
4.4
anthranilate
mutant D83G/F149S, in the presence of 0.05 mM MgCl2, 50 mM Tris-HCl, pH 7.2, at 37°C
5.1
anthranilate
60°C, pH 7.5, 0.05 mM Mg2+, mutant enzyme H107A
5.6
anthranilate
60°C, pH 7.5, 2 mM Mg2+,mutant enzyme E224Q
6
anthranilate
60°C, pH 7.5, 2 mM Mg2+,mutant enzyme D223N
13.3
anthranilate
mutant D83G/F149S, in the presence of 2 mM MgCl2, 50 mM Tris-HCl, pH 7.2, at 37°C
additional information
5-phospho-alpha-D-ribose 1-diphosphate
I36E/M47D double mutant, kcat/KM: 0.0019 1/s*microM
additional information
5-phospho-alpha-D-ribose 1-diphosphate
-
I36E/M47D double mutant, kcat/KM: 0.0019 1/s*microM
additional information
5-phospho-alpha-D-ribose 1-diphosphate
wild-type, kcat/KM: 0.0023 1/s*microM
additional information
5-phospho-alpha-D-ribose 1-diphosphate
-
wild-type, kcat/KM: 0.0023 1/s*microM
additional information
anthranilate
I36E/M47D double mutant, kcat/KM: 4.8 1/s*microM
additional information
anthranilate
-
I36E/M47D double mutant, kcat/KM: 4.8 1/s*microM
additional information
anthranilate
wild-type, kcat/KM: 4.8 1/s*microM
additional information
anthranilate
-
wild-type, kcat/KM: 4.8 1/s*microM
additional information
additional information
single mutants I36E and M47D show the same kcat as I36E/M47D double mutant within experimental error
-
additional information
additional information
-
single mutants I36E and M47D show the same kcat as I36E/M47D double mutant within experimental error
-