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Literature summary for 2.4.2.18 extracted from
- Stabilization of a metabolic enzyme by library selection in Thermus thermophilus (2011), Chembiochem, 12, 1581-1588.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D83G/F149S | mutation leads to monomerization, apparent melting temperature is 9.5°C lower than the wild-type value | Saccharolobus solfataricus |
| I36E/M47D | mutation leads to monomerization, apparent melting temperature is 11.5°C lower than the wild-type value | Saccharolobus solfataricus |
| I36E/M47D/D83G/F149S | mutation leads to monomerization, apparent melting temperature is 21.4°C lower than the wild-type value. kcat/Km for anthranilate is 14.5fold lower than kcat/Km for wild-type enzyme. kcat/Km for anthranilate is lower than kcat/Km for wild-type enzyme. kcat/Km for 5-phospho-alpha-D-ribose 1-diphosphate is 33fold higher than kcat/Km for wild-type enzyme | Saccharolobus solfataricus |
| I36E/M47D/D83G/F149S/F193S | mutation leads to monomerization, apparent melting temperature is 17.4°C lower than the wild-type value. kcat/Km for anthranilate is 52fold lower than kcat/Km for wild-type enzyme. kcat/Km for anthranilate is lower than kcat/Km for wild-type enzyme. kcat/Km for 5-phospho-alpha-D-ribose 1-diphosphate is 31.3fold higher than kcat/Km for wild-type enzyme | Saccharolobus solfataricus |
| I36E/M47D/D83G/F149S/I169T | mutation leads to monomerization, apparent melting temperature is 20.6°C lower than the wild-type value. kcat/Km for anthranilate is 8.9fold lower than kcat/Km for wild-type enzyme. kcat/Km for anthranilate is lower than kcat/Km for wild-type enzyme. kcat/Km for 5-phospho-alpha-D-ribose 1-diphosphate is 24.3fold higher than kcat/Km for wild-type enzyme | Saccharolobus solfataricus |
| I36E/M47D/D83G/F149S/L320M | mutation leads to monomerization, apparent melting temperature is 20.9°C lower than the wild-type value. kcat/Km for anthranilate is 11fold lower than kcat/Km for wild-type enzyme. kcat/Km for anthranilate is lower than kcat/Km for wild-type enzyme. kcat/Km for 5-phospho-alpha-D-ribose 1-diphosphate is 31.3fold higher than kcat/Km for wild-type enzyme | Saccharolobus solfataricus |
| I36E/M47D/D83G/N109S/F149S | mutation leads to monomerization, apparent melting temperature is 20.5°C lower than the wild-type value. kcat/Km for anthranilate is 39fold lower than kcat/Km for wild-type enzyme. kcat/Km for anthranilate is lower than kcat/Km for wild-type enzyme. kcat/Km for 5-phospho-alpha-D-ribose 1-diphosphate is 10fold higher than kcat/Km for wild-type enzyme | Saccharolobus solfataricus |
| I36E/M47D/D83G/N109S/F149S/I169T/L320M/N324I | mutation leads to monomerization, apparent melting temperature is 18.5°C lower than the wild-type value | Saccharolobus solfataricus |
| I36E/M47D/T77I/D83G/F149S | mutation leads to monomerization, apparent melting temperature is 13.3°C lower than the wild-type value. kcat/Km for anthranilate is 13.3fold lower than kcat/Km for wild-type enzyme. kcat/Km for anthranilate is lower than kcat/Km for wild-type enzyme. kcat/Km for 5-phospho-alpha-D-ribose 1-diphosphate is 10fold higher than kcat/Km for wild-type enzyme | Saccharolobus solfataricus |
| I36E/M47D/T77I/D83G/F149S/F193S | mutation leads to monomerization, apparent melting temperature is 11.4°C lower than the wild-type value | Saccharolobus solfataricus |
| I36E/M47D/T77I/D83G/F149S/I169T/F193S/L320M | mutation leads to monomerization, apparent melting temperature is 9.1°C lower than the wild-type value. kcat/Km for anthranilate is 85.7fold lower than kcat/Km for wild-type enzyme. kcat/Km for anthranilate is lower than kcat/Km for wild-type enzyme. kcat/Km for 5-phospho-alpha-D-ribose 1-diphosphate is 2.7fold lower than kcat/Km for wild-type enzyme | Saccharolobus solfataricus |
| I36E/M47D/T77I/D83G/F149S/N109S/I169T/F193S/L320M | mutation leads to monomerization, apparent melting temperature is 8.7°C lower than the wild-type value. kcat/Km for anthranilate is 209fold lower than kcat/Km for wild-type enzyme. kcat/Km for anthranilate is lower than kcat/Km for wild-type enzyme. kcat/Km for 5-phospho-alpha-D-ribose 1-diphosphate is higher than kcat/Km for wild-type enzyme | Saccharolobus solfataricus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.007 | - |
anthranilate | mutant enzyme I36E/M47D/D83G/F149S/I169T, pH 7.2, 37°C | Saccharolobus solfataricus |  |
| 0.008 | - |
anthranilate | mutant enzyme I36E/M47D/D83G/F149S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 0.011 | - |
anthranilate | mutant enzyme I36E/M47D/D83G/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 0.029 | - |
anthranilate | mutant enzyme I36E/M47D/D83G/F149S/F193S, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 0.033 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/D83G/N109S/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 0.037 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/D83G/F149S/F193S, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 0.037 | - |
anthranilate | mutant enzyme I36E/M47D/T77I/D83G/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 0.045 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/D83G/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 0.047 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/T77I/D83G/F149S/N109S/I169T/F193S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 0.051 | - |
anthranilate | mutant enzyme I36E/M47D/T77I/D83G/F149S/N109S/I169T/F193S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 0.054 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/D83G/F149S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 0.055 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/T77I/D83G/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 0.055 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/T77I/D83G/F149S/I169T/F193S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 0.06 | - |
anthranilate | mutant enzyme I36E/M47D/D83G/N109S/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 0.062 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/D83G/F149S/I169T, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 0.082 | - |
anthranilate | mutant enzyme I36E/M47D/T77I/D83G/F149S/I169T/F193S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | Saccharolobus solfataricus | catalyzes the third step in tryptophan biosynthesis | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | Saccharolobus solfataricus P2 | catalyzes the third step in tryptophan biosynthesis | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | P50384 | - |
- |
| Saccharolobus solfataricus P2 | P50384 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Saccharolobus solfataricus | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | catalyzes the third step in tryptophan biosynthesis | Saccharolobus solfataricus | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Saccharolobus solfataricus P2 | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
| anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate | catalyzes the third step in tryptophan biosynthesis | Saccharolobus solfataricus P2 | N-(5-phospho-D-ribosyl)-anthranilate + diphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| sAnPRT | - |
Saccharolobus solfataricus |
| SSO0890 | locus name | Saccharolobus solfataricus |
| strpD | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Saccharolobus solfataricus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.046 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/T77I/D83G/F149S/I169T/F193S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 0.046 | - |
anthranilate | mutant enzyme I36E/M47D/T77I/D83G/F149S/I169T/F193S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 0.78 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/D83G/N109S/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 0.78 | - |
anthranilate | mutant enzyme I36E/M47D/D83G/N109S/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 1.2 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/T77I/D83G/F149S/N109S/I169T/F193S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 1.2 | - |
anthranilate | mutant enzyme I36E/M47D/T77I/D83G/F149S/N109S/I169T/F193S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 1.3 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/T77I/D83G/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 1.3 | - |
anthranilate | mutant enzyme I36E/M47D/T77I/D83G/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 2.7 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/D83G/F149S/F193S, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 2.7 | - |
anthranilate | mutant enzyme I36E/M47D/D83G/F149S/F193S, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 3.4 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/D83G/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 3.4 | - |
anthranilate | mutant enzyme I36E/M47D/D83G/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 3.5 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/D83G/F149S/I169T, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 3.5 | - |
anthranilate | mutant enzyme I36E/M47D/D83G/F149S/I169T, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 3.9 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/D83G/F149S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 3.9 | - |
anthranilate | mutant enzyme I36E/M47D/D83G/F149S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Saccharolobus solfataricus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | catalyzes the third step in tryptophan biosynthesis | Saccharolobus solfataricus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.56 | - |
anthranilate | mutant enzyme I36E/M47D/T77I/D83G/F149S/I169T/F193S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 0.84 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/T77I/D83G/F149S/I169T/F193S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 13 | - |
anthranilate | mutant enzyme I36E/M47D/D83G/N109S/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 23 | - |
anthranilate | mutant enzyme I36E/M47D/T77I/D83G/F149S/N109S/I169T/F193S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 24 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/D83G/N109S/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 24 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/T77I/D83G/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 25 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/T77I/D83G/F149S/N109S/I169T/F193S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 56 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/D83G/F149S/I169T, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 72 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/D83G/F149S/F193S, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 72 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/D83G/F149S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 76 | - |
5-phospho-alpha-D-ribose 1-diphosphate | mutant enzyme I36E/M47D/D83G/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 92 | - |
anthranilate | mutant enzyme I36E/M47D/D83G/F149S/F193S, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 330 | - |
anthranilate | mutant enzyme I36E/M47D/D83G/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 360 | - |
anthranilate | mutant enzyme I36E/M47D/T77I/D83G/F149S, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 460 | - |
anthranilate | mutant enzyme I36E/M47D/D83G/F149S/L320M, pH 7.2, 37°C | Saccharolobus solfataricus | |
| 540 | - |
anthranilate | mutant enzyme I36E/M47D/D83G/F149S/I169T, pH 7.2, 37°C | Saccharolobus solfataricus | |
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