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2.3.1.136: polysialic-acid O-acetyltransferase

This is an abbreviated version!
For detailed information about polysialic-acid O-acetyltransferase, go to the full flat file.

Word Map on EC 2.3.1.136

Reaction

acetyl-CoA
+
an alpha-2,8-linked polymer of sialic acid
=
CoA
 +
polysialic acid acetylated at O-7 or O-9

Synonyms

lecithin:retinol acyltransferase, LRAT, NeuO, OatC, OatWY, polySia specific O-acetyltransferase, polysialic acid O-acetyltransferase, polysialic acid O-AcTase, polysialic acid specific O-acetyltransferase, polysialic acid-specific O-acetyltransferase, polysialic-acid O-acetyltransferase

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.136 polysialic-acid O-acetyltransferase

Engineering

Engineering on EC 2.3.1.136 - polysialic-acid O-acetyltransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H119A
-
complete loss of enzymatic activity without affecting the oligomerization state
NeuO + 12 hepta-nucleotide repeats
-
a set of NeuO alleles with N-terminal 0, 12, 24, and 36 hepta-nucleotide repeats are generated: protein yield: 3.7 mg/L, Km (mM): 0.22 (acetyl-CoA), 1.1 (colomnic acid), kcat (1/sec): 0.56 (acetyl-CoA), 1.85 (colomnic acid)
NeuO + 24 hepta-nucleotide repeats
-
a set of NeuO alleles with N-terminal 0, 12, 24, and 36 hepta-nucleotide repeats are generated: protein yield: 5.0 mg/L, Km (mM): 0.27 (acetyl-CoA), 1.0 (colomnic acid), kcat (1/sec): 1.07 (acetyl-CoA), 2.57 (colomnic acid)
NeuO + 36 hepta-nucleotide repeats
-
a set of NeuO alleles with N-terminal 0, 12, 24, and 36 hepta-nucleotide repeats are generated: protein yield: 5.0 mg/L, Km (mM): 0.22 (acetyl-CoA), 0.22 (propionyl-CoA) 1.6 (colomnic acid), kcat (1/sec): 1.16 (acetyl-CoA), 0.09 (propionyl-CoA), 3.84 (colomnic acid)
W143A
-
complete loss of enzymatic activity without affecting the oligomerization state
D359A
-
mutant shows enzymatic activity comparable to wild-type
D371A
-
mutant shows enzymatic activity comparable to wild-type
D376A
-
mutant shows no enzymatic activity
DELTA1-103
-
mutant lacking the first 103 amino acids: after purification mutant is found as a monomer, indicating that dimerization of OatC depends on the first 34 amino acids. Mutant shows no activity
DELTA1-34
-
mutant lacking the first 34 amino acids: after purification mutant is found as a monomer, indicating that dimerization of OatC depends on the first 34 amino acids. Mutant retains 25% activity
E336A
-
mutant shows enzymatic activity comparable to wild-type
E345A
-
mutant shows enzymatic activity comparable to wild-type
E379A
-
mutant shows enzymatic activity comparable to wild-type
H111A
-
mutant shows enzymatic activity comparable to wild-type
H121A
mutant shows dramatically reduced activity
H183A
-
mutant shows enzymatic activity comparable to wild-type
H267A
-
mutant shows enzymatic activity comparable to wild-type
H31A
-
mutant shows enzymatic activity comparable to wild-type
H399A
-
mutant shows no enzymatic activity
H456A
-
mutant shows reduced enzymatic activity, 70% of wild-type level
S285A
-
mutant retains 80% of wild-type activity
S286A
-
mutant shows no enzymatic activity
S286C
-
mutant shows no enzymatic activity
W145A
mutant shows dramatically reduced activity
Y171A
mutant shows dramatically reduced activity
additional information