Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Neisseria meningitidis |
Crystallization (Comment) | Organism |
---|---|
OatWY is crystalised with bound substrate acetyl-CoA and its analogs (CoA, S-(2-oxopropyl)-CoA) using the hanging drop vapor diffusion technique. The structure of OatWY reveals an intimate homotrimer of left-handed beta-helix motifs that frame a deep active site cleft selective for the polysialic acid-bearing substrate. A significant movement of Tyr-171 blocks the active site of the enzyme in its native form | Neisseria meningitidis |
Protein Variants | Comment | Organism |
---|---|---|
H121A | mutant shows dramatically reduced activity | Neisseria meningitidis |
W145A | mutant shows dramatically reduced activity | Neisseria meningitidis |
Y171A | mutant shows dramatically reduced activity | Neisseria meningitidis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
([Glc-(alpha1-4)-Sia]n) | - |
Neisseria meningitidis | |
0.21 | - |
acetyl-CoA | - |
Neisseria meningitidis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neisseria meningitidis | O33391 | - |
- |
Purification (Comment) | Organism |
---|---|
using affnitiy chromatography and ion-exchange chromatography | Neisseria meningitidis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + ([Glc-(alpha1-4)-Sia]) | relative activity: 19.2% | Neisseria meningitidis | ? | - |
? | |
acetyl-CoA + ([Glc-(alpha1-4)-Sia]n) | relative activity: 100% | Neisseria meningitidis | ? | - |
? | |
acetyl-CoA + colominic acid | relative activity: 2.1% | Neisseria meningitidis | ? | - |
? | |
additional information | when S-(2-oxopropyl)-CoA is used as a donor activity is dramatically reduced | Neisseria meningitidis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | crystal structure | Neisseria meningitidis |
Synonyms | Comment | Organism |
---|---|---|
OatWY | - |
Neisseria meningitidis |
polysialic-acid O-acetyltransferase | - |
Neisseria meningitidis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Neisseria meningitidis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
acetyl-CoA | - |
Neisseria meningitidis | |
2.1 | - |
([Glc-(alpha1-4)-Sia]n) | - |
Neisseria meningitidis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Neisseria meningitidis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.3 | - |
acetyl-CoA | - |
Neisseria meningitidis | |
190 | - |
([-6)-Glc-(alpha1-4)-Sia-(alpha2-]n) oder ([-6)-Glc-(alpha1-4)-Sia-(alpha2-]n) | - |
Neisseria meningitidis |