1.14.13.225: F-actin monooxygenase
This is an abbreviated version!
For detailed information about F-actin monooxygenase, go to the full flat file.
Word Map on EC 1.14.13.225
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1.14.13.225
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plexins
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calponin
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semaphorin-plexin
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sema3a
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plexa
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semaphorin3a
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rab8
- 1.14.13.225
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plexins
- calponin
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semaphorin-plexin
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sema3a
- plexa
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semaphorin3a
- rab8
Reaction
Synonyms
MICAL, MICAL-1, MICAL-2, MICAL1, Mical2, MICAL2PV, MICAL3
ECTree
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Substrates Products
Substrates Products on EC 1.14.13.225 - F-actin monooxygenase
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REACTION DIAGRAM
[F-actin]-L-methionine + NADH + O2 + H+
[F-actin]-L-methionine-(R)-S-oxide + NAD+ + H2O
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[F-actin]-L-methionine-(R)-S-oxide + NADP+ + H2O
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Mical post-translationally oxidizes the methionine 44 residue within the D-loop of actin, simultaneously severing filaments and decreasing polymerization
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[F-actin]-L-methionine + NADPH + O2 + H+
[F-actin]-L-methionine-(R)-S-oxide + NADP+ + H2O
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[F-actin]-L-methionine + NADPH + O2 + H+
[F-actin]-L-methionine-(R)-S-oxide + NADP+ + H2O
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[F-actin]-L-methionine + NADPH + O2 + H+
[F-actin]-L-methionine-(R)-S-oxide + NADP+ + H2O
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only 2-3 residues are oxidized in each actin monomer. Several residues, including Met44 and Met47, are modified with similar probability
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[F-actin]-L-methionine + NADPH + O2 + H+
[F-actin]-L-methionine-(R)-S-oxide + NADP+ + H2O
usage of F-actin from rabbit skeletal muscle purified from G-actin by ultracentrifugation
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[F-actin]-L-methionine + NADPH + O2 + H+
[F-actin]-L-methionine-(R)-S-oxide + NADP+ + H2O
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[F-actin]-L-methionine + NADPH + O2 + H+
[F-actin]-L-methionine-(R)-S-oxide + NADP+ + H2O
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the Km for NADPH of the NADPH oxidase reaction is sensitive to ionic strength and type of ions. The apparent kcat (pH 7) is limited by enzyme reduction by NADPH, which occurs without detectable intermediates. The reaction is also sensitive to solvent viscosity
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additional information
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the Km for NADPH of the NADPH oxidase reaction is sensitive to ionic strength and type of ions. The apparent kcat (pH 7) is limited by enzyme reduction by NADPH, which occurs without detectable intermediates. The reaction is also sensitive to solvent viscosity
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additional information
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MICAL has additionally NADPH oxidase activity that underlies F-actin disassembly simultaneously with the oxidation of NADPH. For MICAL3 containing the FMO and CH domains, the kcat/Km ratio for NADPH oxidase activity increases dramatically on adding F-actin, while the kcat/Km value for MICAL3 lacking the CH domain changes little
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additional information
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MICAL has additionally NADPH oxidase activity that underlies F-actin disassembly simultaneously with the oxidation of NADPH. For MICAL3 containing the FMO and CH domains, the kcat/Km ratio for NADPH oxidase activity increases dramatically on adding F-actin, while the kcat/Km value for MICAL3 lacking the CH domain changes little
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additional information
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the reductive half-reaction of the mical2 hydroxylase domain is stimulated by F-actin. In the absence of actin, NADPH reduces the flavin relatively slowly. Actin speeds that reaction significantly. The separate monooxygenase domain has the classic regulatory behavior of flavin-dependent aromatic hydroxylases (Class A monooxygenases) with slow reduction of the flavin when the substrate to be oxygenated is absent
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