Activating Compound | Comment | Organism | Structure |
---|---|---|---|
F-actin | stimulates NADPH oxidation by increasing kcat and kcat/KNADPH about 5 and 200fold, respectively | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glycerol | 10%, 2fold increase in Km for NADPH | Homo sapiens | |
NaCl | 0.1 M, 20fold increase in Km for NADPH | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
NADPH | the Km for NADPH of the NADPH oxidase reaction is sensitive to ionic strength and type of ions. pH 7.0, 25°C | Homo sapiens | |
0.028 | - |
NADPH | pH 7.0, 25°C | Homo sapiens | |
0.093 | - |
NADPH | pH 7.0, 25°C, presence of 10% glycerol | Homo sapiens | |
0.499 | - |
NADPH | pH 7.0, 25°C, presence of 0.1 M NaCl | Homo sapiens | |
0.58 | - |
NADH | pH 7.0, 25°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q8TDZ2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the Km for NADPH of the NADPH oxidase reaction is sensitive to ionic strength and type of ions. The apparent kcat (pH 7) is limited by enzyme reduction by NADPH, which occurs without detectable intermediates. The reaction is also sensitive to solvent viscosity | Homo sapiens | ? | - |
? | |
[F-actin]-L-methionine + NADH + O2 + H+ | - |
Homo sapiens | [F-actin]-L-methionine-(R)-S-oxide + NAD+ + H2O | - |
? | |
[F-actin]-L-methionine + NADPH + O2 + H+ | - |
Homo sapiens | [F-actin]-L-methionine-(R)-S-oxide + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MICAL-1 | - |
Homo sapiens |
MICAL1 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
NADH | pH 7.0, 25°C | Homo sapiens | |
2.6 | - |
NADPH | pH 7.0, 25°C, presence of 0.1 M NaCl | Homo sapiens | |
2.9 | - |
NADPH | pH 7.0, 25°C, presence of 10% glycerol | Homo sapiens | |
4 | - |
NADPH | pH 7.0, 25°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | NADPH is 10fold preferred over NADH | Homo sapiens | |
NADPH | 10fold preferred over NADH | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.48 | - |
NADH | pH 7.0, 25°C | Homo sapiens | |
5.2 | - |
NADPH | pH 7.0, 25°C, presence of 0.1 M NaCl | Homo sapiens | |
31.2 | - |
NADPH | pH 7.0, 25°C, presence of 10% glycerol | Homo sapiens | |
143 | - |
NADPH | pH 7.0, 25°C | Homo sapiens |