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Literature summary for 1.14.13.225 extracted from

  • Zucchini, D.; Caprini, G.; Pasterkamp, R.J.; Tedeschi, G.; Vanoni, M.A.
    Kinetic and spectroscopic characterization of the putative monooxygenase domain of human MICAL-1 (2011), Arch. Biochem. Biophys., 515, 1-13.
    View publication on PubMed
Search for more literature for 1.14.13.225

Activating Compound

Activating Compound Comment Organism Structure
F-actin stimulates NADPH oxidation by increasing kcat and kcat/KNADPH about 5 and 200fold, respectively Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
glycerol 10%, 2fold increase in Km for NADPH Homo sapiens
NaCl 0.1 M, 20fold increase in Km for NADPH Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 NADPH the Km for NADPH of the NADPH oxidase reaction is sensitive to ionic strength and type of ions. pH 7.0, 25°C Homo sapiens
0.028
-
 NADPH pH 7.0, 25°C Homo sapiens
0.093
-
 NADPH pH 7.0, 25°C, presence of 10% glycerol Homo sapiens
0.499
-
 NADPH pH 7.0, 25°C, presence of 0.1 M NaCl Homo sapiens
0.58
-
 NADH pH 7.0, 25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8TDZ2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the Km for NADPH of the NADPH oxidase reaction is sensitive to ionic strength and type of ions. The apparent kcat (pH 7) is limited by enzyme reduction by NADPH, which occurs without detectable intermediates. The reaction is also sensitive to solvent viscosity Homo sapiens ?
-
 ?
[F-actin]-L-methionine + NADH + O2 + H+
-
 Homo sapiens [F-actin]-L-methionine-(R)-S-oxide + NAD+ + H2O
-
 ?
[F-actin]-L-methionine + NADPH + O2 + H+
-
 Homo sapiens [F-actin]-L-methionine-(R)-S-oxide + NADP+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
MICAL-1
-
 Homo sapiens
MICAL1
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.28
-
 NADH pH 7.0, 25°C Homo sapiens
2.6
-
 NADPH pH 7.0, 25°C, presence of 0.1 M NaCl Homo sapiens
2.9
-
 NADPH pH 7.0, 25°C, presence of 10% glycerol Homo sapiens
4
-
 NADPH pH 7.0, 25°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADH NADPH is 10fold preferred over NADH Homo sapiens
NADPH 10fold preferred over NADH Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.48
-
 NADH pH 7.0, 25°C Homo sapiens
5.2
-
 NADPH pH 7.0, 25°C, presence of 0.1 M NaCl Homo sapiens
31.2
-
 NADPH pH 7.0, 25°C, presence of 10% glycerol Homo sapiens
143
-
 NADPH pH 7.0, 25°C Homo sapiens