EC Number   |
Protein Variants |
Reference |
|---|
    5.1.1.3 | A151V |
production by site-directed mutagenesis, alanine ist essential for activity, mutation of residue 151 located at the entryway to the active site reveals that FnGR is very sensitive to increased steric bulk at this position |
701927 |
| 5.1.1.3 | A75T |
Ki: 0.661 mM (inhibitor: D-glutamate). Turnover number: 1.78/sec (substrate: L-glutamate), 0.065/sec (substrate: D-glutamate). Km: 7.4 mM (substrate: L-glutamate), Km: 0.275 mM (substrate: D-glutamate) |
676152 |
| 5.1.1.3 | C184A |
mutant C73A and C184A enzymes are inactive as racemases. However they are capable of catalyzing the elimination of HCl from opposite enantiomers of threo-3-chloroglutamic acid, a process that presumably requires only one enzymic base. It appears that Cys73 is responsible for the abstraction of the C-2 hydrogen from R-Glu and Cys184 abstracts the proton from S-glutamate in the racemization reaction of the wild type enzyme |
2116 |
| 5.1.1.3 | C185A |
predicted active site is mutated by site-directed mutagenesis, detectable racemase activity is attenuated by at least 100fold |
674336 |
| 5.1.1.3 | C185S |
decrease in racemization activity, mutant retains its gyrase inhibition ability |
694039 |
| 5.1.1.3 | C188A |
predicted active site is mutated by site-directed mutagenesis, detectable racemase activity is attenuated by at least 100fold |
674336 |
| 5.1.1.3 | C73A |
mutant C73A and C184A enzymes are inactive as racemases. However they are capable of catalyzing the elimination of HCl from opposite enantiomers of threo-3-chloroglutamic acid, a process that presumably requires only one enzymic base. It appears that Cys73 is responsible for the abstraction of the C-2 hydrogen from R-Glu and Cys184 abstracts the proton from S-glutamate in the racemization reaction of the wild type enzyme |
2116 |
| 5.1.1.3 | C74A |
predicted active site is mutated by site-directed mutagenesis, detectable racemase activity is attenuated by at least 100fold |
674336 |
| 5.1.1.3 | C75S |
decrease in racemization activity, mutant retains its gyrase inhibition ability |
694039 |
| 5.1.1.3 | C75SC185S |
less than 10% of wild-type activity, mutant retains its gyrase inhibition ability |
694039 |
