EC Number   |
Protein Variants |
Reference  |
|---|
   3.2.1.135 | more |
diverse mutants are constructed and specific activities, sugar compositions of pullulan hydrolysate and hydrolysis activities toward alpha-(1-4) and alpha-(1-6)-glucosidic linkages analyzed |
-, 393353 |
| 3.2.1.135 | I358V |
mutation decreases the preference for alpha(1-6)-branched oligosaccharides and pullulan as substrates |
-, 646815 |
| 3.2.1.135 | I358W |
mutation reduces the acceptability of alpha(1-6)-branched oligo- and polysaccharides |
-, 646815 |
| 3.2.1.135 | M375L |
mutation increases transglycosylation activity in comparison to wild-type enzyme |
-, 646815 |
| 3.2.1.135 | S422V |
mutation increases transglycosylation activity in comparison to wild-type enzyme |
-, 646815 |
| 3.2.1.135 | Y377D |
mutation decreases transglycosylation activity in comparison to wild-type enzyme |
646815 |
| 3.2.1.135 | Y377F |
mutation increases transglycosylation activity in comparison to wild-type enzyme |
-, 646815 |
| 3.2.1.135 | Y377S |
mutation decreases transglycosylation activity in comparison to wild-type enzyme |
646815 |
| 3.2.1.135 | D328H |
site-directed mutagenesis, inactive mutant |
664673 |
| 3.2.1.135 | D328N |
site-directed mutagenesis, inactive mutant |
664673 |
