EC Number |
Protein Variants |
Reference |
---|
3.2.1.135 | A416I |
t1/2 of mutant enzyme at 70°C is 17 min, compared to 15 min for wild-type enzyme. Mutation does not compromise the catalytic activity |
752088 |
3.2.1.135 | A566L |
t1/2 of mutant enzyme at 70°C is 27 min, compared to 15 min for wild-type enzyme. Mutation does not compromise the catalytic activity |
752088 |
3.2.1.135 | D328H |
site-directed mutagenesis, inactive mutant |
664673 |
3.2.1.135 | D328N |
site-directed mutagenesis, inactive mutant |
664673 |
3.2.1.135 | D424H |
site-directed mutagenesis, inactive mutant |
664673 |
3.2.1.135 | D424N |
site-directed mutagenesis, inactive mutant |
664673 |
3.2.1.135 | D46E |
t1/2 of mutant enzyme at 70°C is 100 min, compared to 15 min for wild-type enzyme. Mutation does not compromise the catalytic activity |
752088 |
3.2.1.135 | E357H |
site-directed mutagenesis, inactive mutant |
664673 |
3.2.1.135 | E357Q |
site-directed mutagenesis, inactive mutant |
664673 |
3.2.1.135 | I358V |
mutation decreases the preference for alpha(1-6)-branched oligosaccharides and pullulan as substrates |
-, 646815 |