EC Number   |
Protein Variants |
Reference |
|---|
    3.1.3.25 | C150S |
insensitive toward inactivation with oxygen or thioredoxin, no effect on activity |
-, 653807 |
| 3.1.3.25 | D104N |
activity almost completely abolished |
650150 |
| 3.1.3.25 | D104N |
IMPA2 mutant, no activity |
680880 |
| 3.1.3.25 | D107N |
activity almost completely abolished |
650150 |
| 3.1.3.25 | D221A |
loss of 60-70% of activity for both FBPase and IMPase activities |
-, 749477 |
| 3.1.3.25 | D235N |
activity almost completely abolished |
650150 |
| 3.1.3.25 | D26A |
site-directed mutagenesis, the mutant shows no significant change in the Mg2+ requirement for optimal activity |
716857 |
| 3.1.3.25 | D38A |
site-directed mutagenesis, the mutant shows an increased Km for Mg2+, but little effect on kcat compared to the wild-type enzyme, the mutant has a dramatically enhanced sensitivity to Li+ compared to the wild-type, with an IC50 of 12 mM. Electron density map for MJ0109 D38A mobile loop and active site with and without bound Li+, overview |
716857 |
| 3.1.3.25 | D40N |
site-sirected mutagenesis, nearly inactive mutant concerning both inositol monophosphatase and fructose-1,6-bisphosphatase activities |
-, 663892 |
| 3.1.3.25 | D460N |
loss of activity with phosphatidylinositol 4-phosphate |
751172 |
