EC Number   |
Protein Variants |
Reference |
|---|
   3.1.3.23 | A69M |
the mutation slightly affects the substrate specificity |
730667 |
| 3.1.3.23 | G12D |
strain IQB849 carrying a single-base substitution C to A introduced in the hairpin region displaying an augment in araL'-'lacZ expression of about 30fold in the presence of inducer, the point mutation increases the free energy of the mRNA secondary structure |
714982 |
| 3.1.3.23 | H72K |
the mutant is inactive in phosphomonoester dephosphorylation |
730667 |
| 3.1.3.23 | I68M |
the mutation slightly affects the substrate specificity |
730667 |
| 3.1.3.23 | I68M/A69M/K71R/H72K |
the mutant is inactive in phosphomonoester dephosphorylation |
730667 |
| 3.1.3.23 | K71R |
the mutation slightly affects the substrate specificity |
730667 |
| 3.1.3.23 | more |
a translational fusion of the 5'-end of the araL gene to the lacZ reporter gene from Escherichia coli is constructed and integrated into the Bacillus subtilis chromosome. The construct comprises the araL ribosome-binding site, the initiation codon and a fusion between codon 10 of araL and codon 7 of Escherichia coli lacZ |
714982 |
| 3.1.3.23 | N70S |
the mutant shows wild type activity |
730458 |
| 3.1.3.23 | T9K/G12D |
the double point mutation, C to A and G to T, introduced a compensatory T in the other part of the stem, thus regenerating the stem-loop structure in strain IQB857 and drastically reducing the expression of araL'-'lacZ |
714982 |
