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Literature summary for 3.1.3.23 extracted from

  • Caparros-Martin, J.A.; McCarthy-Suarez, I.; Culianez-Macia, F.A.
    The kinetic analysis of the substrate specificity of motif 5 in a HAD hydrolase-type phosphosugar phosphatase of Arabidopsis thaliana (2014), Planta, 240, 479-487.
    View publication on PubMed
Search for more literature for 3.1.3.23

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli TB1 cells Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
A69M the mutation slightly affects the substrate specificity Arabidopsis thaliana
H72K the mutant is inactive in phosphomonoester dephosphorylation Arabidopsis thaliana
I68M the mutation slightly affects the substrate specificity Arabidopsis thaliana
I68M/A69M/K71R/H72K the mutant is inactive in phosphomonoester dephosphorylation Arabidopsis thaliana
K71R the mutation slightly affects the substrate specificity Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6
-
 D-ribose 5-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.8
-
 D-ribose 5-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
4.1
-
 D-ribose 5-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
4.6
-
 2-deoxy-D-glucose 6-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
4.9
-
 D-mannose 6-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
5
-
 D-mannose 6-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
5
-
 2-deoxy-D-glucose 6-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
5
-
 2-deoxy-D-glucose 6-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
5.5
-
 D-mannose 6-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
5.9
-
 D-ribose 5-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
5.9
-
 2-deoxy-D-glucose 6-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
6.7
-
 D-mannose 6-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
6.7
-
 D-glucose 6-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
7.1
-
 DL-glycerol 3-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
7.1
-
 D-glucose 6-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
7.3
-
 DL-glycerol 3-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
7.7
-
 D-glucose 6-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
7.7
-
 D-fructose 6-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
8
-
 D-glucose 6-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
8
-
 D-fructose 6-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
8
-
 D-fructose 6-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
8
-
 DL-glycerol 3-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
8.3
-
 DL-glycerol 3-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
9.1
-
 D-fructose 6-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42700
-
 x * 42700, maltose-binding protein-bound enzyme, SDS-PAGE Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9ZVJ5
-
-

Purification (Commentary)

Purification (Comment) Organism
amylose resin column chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-Deoxy-D-glucose 6-phosphate + H2O preferred substrate Arabidopsis thaliana 2-Deoxy-D-glucose + phosphate
-
 ?
2-deoxy-D-ribose 5-phosphate + H2O
-
 Arabidopsis thaliana 2-deoxy-D-ribose + phosphate
-
 ?
D-fructose 1-phosphate + H2O
-
 Arabidopsis thaliana D-fructose + phosphate
-
 ?
D-fructose 6-phosphate + H2O
-
 Arabidopsis thaliana D-fructose + phosphate
-
 ?
D-glucose 6-phosphate + H2O
-
 Arabidopsis thaliana D-glucose + phosphate
-
 ?
D-mannose 6-phosphate + H2O preferred substrate Arabidopsis thaliana D-mannose + phosphate
-
 ?
D-ribose 5-phosphate + H2O preferred substrate Arabidopsis thaliana D-ribose + phosphate
-
 ?
DL-glycerol 3-phosphate + H2O
-
 Arabidopsis thaliana glycerol + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 42700, maltose-binding protein-bound enzyme, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
phosphosugar phosphatase
-
 Arabidopsis thaliana
Sgpp
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6
-
 D-fructose 6-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
6.6
-
 DL-glycerol 3-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
7.1
-
 D-glucose 6-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
19
-
 D-fructose 6-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
19
-
 D-fructose 6-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
19
-
 D-fructose 6-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
22
-
 DL-glycerol 3-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
23
-
 DL-glycerol 3-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
24
-
 DL-glycerol 3-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
24
-
 D-glucose 6-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
24
-
 D-glucose 6-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
26
-
 D-mannose 6-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
26
-
 D-glucose 6-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
31
-
 2-deoxy-D-glucose 6-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
32
-
 D-mannose 6-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
32
-
 D-mannose 6-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
33
-
 D-ribose 5-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
33
-
 D-mannose 6-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
33
-
 2-deoxy-D-glucose 6-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
33
-
 2-deoxy-D-glucose 6-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
34
-
 2-deoxy-D-glucose 6-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
39
-
 D-ribose 5-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
39
-
 D-ribose 5-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
39
-
 D-ribose 5-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.4
-
 D-fructose 6-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
0.8
-
 DL-glycerol 3-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
0.9
-
 D-glucose 6-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
2.4
-
 D-fructose 6-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
2.4
-
 D-fructose 6-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
2.5
-
 D-fructose 6-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
2.8
-
 DL-glycerol 3-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.1
-
 DL-glycerol 3-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.2
-
 D-glucose 6-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.4
-
 DL-glycerol 3-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.4
-
 D-glucose 6-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.8
-
 D-mannose 6-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
3.8
-
 D-glucose 6-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
5.2
-
 2-deoxy-D-glucose 6-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
5.6
-
 D-ribose 5-phosphate mutant enzyme A69M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
5.8
-
 D-mannose 6-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
6.6
-
 D-mannose 6-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
6.6
-
 2-deoxy-D-glucose 6-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
6.7
-
 D-mannose 6-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
6.9
-
 2-deoxy-D-glucose 6-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
7.2
-
 2-deoxy-D-glucose 6-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
9.4
-
 D-ribose 5-phosphate mutant enzyme K71R, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
10.2
-
 D-ribose 5-phosphate mutant enzyme I68M, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana
10.7
-
 D-ribose 5-phosphate wild type enzyme, in 20 mM Tris-HCl(pH 7.0), 5 mM MgCl2, at 32°C Arabidopsis thaliana