EC Number   |
Protein Variants |
Reference |
|---|
    2.7.1.17 | D233A |
loss of activity |
675423 |
| 2.7.1.17 | D6A |
19fold decreased ATPase activity compared to the wild type enzyme |
675423 |
| 2.7.1.17 | DELTAxyl3 |
strain FPL-YS30, D-xylulokinase mutant with deletion of gene xyl3, proposal of an alternative pathway mediating xylose assimilation via arabinitol and ribulose-5-phosphate that bypasses the xylulokinase step |
-, 684529 |
| 2.7.1.17 | more |
construction of efficient xylose-fermenting Saccharomyces cerevisiae strain DXS through a synthetic isozyme system of xylose reductase from Scheffersomyces stipitis. The xylose-metabolic genes XYL1 (EC 1.1.1.307), XYL2 (EC 1.1.1.9), and XYL3 from Scheffersomyces stipitis are introduced into Saccharomyces cerevisiae. Construction of control strains SR6 and MM through random integration of the XR (NADPH) or mXR (NADH) expression cassette, respectively, at the multipled elements of the genome of Saccharomyces cerevisiae strain D452-2. Fermentation parameters of engineered Saccharomyces cerevisiae strains in mixed sugar fermentations (glucose and xylose), overview |
760714 |
| 2.7.1.17 | more |
enzyme disruption mutant, produces under aerobic as well as oxygen-limited conditions a negligible amount of ethanol and converts xylose mainly to xylitol. Xylose consumption increases 5fold under aerobic compared with oxygen-limited conditions, reaching a production of 26g/l of xylitonl |
660778 |
| 2.7.1.17 | more |
Escherichia coli xylulokinase gene xylB is PCR-amplified from Escherichia coli genomic DNA, genes of interest are cloned downstream of a tac promoter and upstream of a transcription termination sequence into the medium copy vector pLOI3809, transforming plasmid into a DELTAxylB mutant E. coli strain (PC07) |
688820 |
| 2.7.1.17 | more |
Pichia stipitis xylulokinase gene (XYL3) is PCR-amplified from Pichia stipitis (strain UC7) genomic DNA, genes of interest are cloned downstream of a tac promoter and upstream of a transcription termination sequence into the medium copy vector pLOI3809, transforming plasmid into a DELTAxylB mutant Escherichia coli strain (PC07), determine whether an alternative xylulokinase (one not expected to act on xylitol) could functionally replace XylB in Escherichia coli, Xyl3 shares only 23% amino acid sequence identity with Escherichia coli XylB |
688819 |
| 2.7.1.17 | more |
solubility of highly expressed mutant enzymes increased 8- to 77fold after introduction of GroEL-GroES into Escherichia coli |
661959 |
