Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain PC07 with deleted xylB-gene and introduced plasmid with Escherichia coli xylB gene | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | Escherichia coli xylulokinase gene xylB is PCR-amplified from Escherichia coli genomic DNA, genes of interest are cloned downstream of a tac promoter and upstream of a transcription termination sequence into the medium copy vector pLOI3809, transforming plasmid into a DELTAxylB mutant E. coli strain (PC07) | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-xylulose | Escherichia coli | - |
ADP + D-xylulose 5-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
DELTAxylB mutant strain (PC07), xylB encodes XK in Escherichia coli | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
3.8 U/mg protein (native substrate 5 mM xylulose), 0.76 U/mg protein (substrate 75 mM xylitol), activities measured from cell lysates by monitoring NADH depletion, using an assay in which pentulokinase activity is coupled to pyruvate kinase and lactate dehydrogenase activities, reaction mixture (final concentrations indicated): 71 mM TrisHCl pH 7.5, 7.1 mM MgCl2, 1 mM EDTA, 50 mM KCl, 7.1 mM KF, 5 mM KCN, 1.4 mM ATP, 1 mM phosphoenolpyruvic acid, 0.3 mM NADH, 0.7 U/ml pyruvate kinase, 1 U/ml lactate dehydrogenase, and 5 mM D-xylulose or 75 mM xylitol | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-xylitol | analyzed with 31P NMR spectrum | Escherichia coli | ADP + D-xylitol-1-phosphate | - |
? | |
ATP + D-xylitol | analyzed with 31P NMR spectrum | Escherichia coli | ADP + D-xylitol-5-phosphate | - |
? | |
ATP + D-xylulose | - |
Escherichia coli | ADP + D-xylulose 5-phosphate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |