EC Number   |
Protein Variants |
Reference |
|---|
    2.5.1.31 | A143V |
the C30 intermediate is formed to a greater extent and is longer lived in the process catalyzed by the A69L mutant |
637493 |
| 2.5.1.31 | A69L |
the small side chain of Ala-69 is required for rapid elongation to the C55 product |
637493 |
| 2.5.1.31 | A72L |
mutation results in shorter ultimate products with C20-35 |
-, 673574 |
| 2.5.1.31 | A72L/F73L |
mutation results in shorter ultimate products with C20-35 |
673574 |
| 2.5.1.31 | A72L/F73L/W78L |
mutation results in shorter ultimate products with C20-35 |
-, 673574 |
| 2.5.1.31 | C234A |
C234A UPPS mutant is crystallized using the hanging drop method, C234A has unchanged kinetic properties compared to wild-type |
699031 |
| 2.5.1.31 | C234A |
kinetic data similar to wild-type. Crystallization data |
699031 |
| 2.5.1.31 | C234A |
kinetik data similar to wild-type. Crystallization data |
699031 |
| 2.5.1.31 | C234A |
site-directed mutagenesis, mutation to prevent intramolecular disulfide bond formed during the long period of crystallization process |
699031 |
| 2.5.1.31 | D150A |
turnover-number is 44% of the activity of the wild-type enzyme, Km value for (2E,6E)-farnesyl diphosphate is equal to that of the wild-type enzyme, Km-value for isopentenyl diphosphate is about 50fold higher than that of the wild-type enzyme |
637495 |
