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Literature summary for 2.5.1.31 extracted from

  • Pan, J.J.; Yang, L.W.; Liang, P.H.
    Effect of site-directed mutagenesis of the conserved aspartate and glutamate on E. coli undecaprenyl pyrophosphate synthase catalysis (2000), Biochemistry, 39, 13856-13861.
    View publication on PubMed
Search for more literature for 2.5.1.31

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Protein Variants

Protein Variants Comment Organism
D150A turnover-number is 44% of the activity of the wild-type enzyme, Km value for farnesyl diphosphate is equal to that of the wild-type enzyme, Km-value for isopentenyl diphosphate is about 50fold higher than that of the wild-type enzyme Escherichia coli
D150A turnover-number is 44% of the activity of the wild-type enzyme, Km value for (2E,6E)-farnesyl diphosphate is equal to that of the wild-type enzyme, Km-value for isopentenyl diphosphate is about 50fold higher than that of the wild-type enzyme Escherichia coli
D190A no significant change Escherichia coli
D218A turnover-number is about 9% of the activity of the wild-type enzyme, Km value for farnesyl diphosphate is equal to that of the wild-type enzyme, Km-value for isopentenyl diphosphate is comparable to that of the wild-type enzyme Escherichia coli
D223A no significant change Escherichia coli
D26A turnover-number is less than 1% of that of the wild-type enzyme, Km value for farnesyl diphosphate is comparable to that of the wild-type enzyme, Km-value for isopentenyl diphosphate is 3.5fold higher than that of the wild-type enzyme Escherichia coli
E198A no significant change Escherichia coli
E213A turnover-number is less than 1% of the activity of the wild-type enzyme, Km value for farnesyl diphosphate is 1.75fold higher than that of the wild-type enzyme, Km-value for isopentenyl diphosphate is about 68.3fold higher than that of the wild-type enzyme Escherichia coli
E73A turnover-number is 12% of that of the wild-type enzyme, Km value for farnesyl diphosphate is equal to that of the wild-type enzyme, Km-value for isopentenyl diphosphate is about 4fold higher than that of the wild-type enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
 farnesyl diphosphate wild-type enzyme, pH 7.5, 25°C Escherichia coli
0.0004
-
 farnesyl diphosphate mutant enzymes E73A, D150A and D218A, pH 7.5, 25°C Escherichia coli
0.0004
-
 (2E,6E)-farnesyl diphosphate mutant enzymes E73A, D150A and D218A, pH 7.5, 25°C; wild-type enzyme, pH 7.5, 25°C Escherichia coli
0.0005
-
 (2E,6E)-farnesyl diphosphate mutant enzyme D26A, pH 7.5, 25°C Escherichia coli
0.0005
-
 farnesyl diphosphate mutant enzyme D26A, pH 7.5, 25°C Escherichia coli
0.0007
-
 (2E,6E)-farnesyl diphosphate mutant enzyme E213A, pH 7.5, 25°C Escherichia coli
0.0007
-
 farnesyl diphosphate mutant enzyme E213A, pH 7.5, 25°C Escherichia coli
0.0041
-
 isopentenyl diphosphate pH 7.5, 25°C Escherichia coli
0.0041
-
 isopentenyl diphosphate pH 7.5, 25°C, wild type Escherichia coli
0.0044
-
 isopentenyl diphosphate mutant enzyme D218A, pH 7.5, 25°C Escherichia coli
0.0141
-
 isopentenyl diphosphate mutant enzyme D26A, pH 7.5, 25°C Escherichia coli
0.0157
-
 isopentenyl diphosphate mutant enzyme R77A, pH 7.5, 25°C Escherichia coli
0.0162
-
 isopentenyl diphosphate
-
 Escherichia coli
0.0162
-
 isopentenyl diphosphate pH 7.5, 25°C, wild type Escherichia coli
0.27
-
 isopentenyl diphosphate mutant enzyme D150A, pH 7.5, 25°C Escherichia coli
0.28
-
 isopentenyl diphosphate mutant enzyme D213A, pH 7.5, 25°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate
-
 Escherichia coli 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate
-
 ?
isopentenyl diphosphate + farnesyl diphosphate
-
 Escherichia coli C55-polyprenyl diphosphate + C50-polyprenyl diphosphate + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
undecaprenyl pyrophosphate synthase
-
 Escherichia coli
UPPs
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Escherichia coli
0.0033
-
 (2E,6E)-farnesyl diphosphate mutant enzyme D26A, pH 7.5, 25°C Escherichia coli
0.026
-
 (2E,6E)-farnesyl diphosphate mutant enzyme E213A, pH 7.5, 25°C Escherichia coli
0.22
-
 (2E,6E)-farnesyl diphosphate mutant enzyme D218A, pH 7.5, 25°C Escherichia coli
0.3
-
 (2E,6E)-farnesyl diphosphate mutant enzymes E73A, pH 7.5, 25°C Escherichia coli
1.1
-
 (2E,6E)-farnesyl diphosphate mutant enzyme D150A, pH 7.5, 25°C Escherichia coli
2.5
-
 (2E,6E)-farnesyl diphosphate wild type, pH 7.5, 25°C Escherichia coli