EC Number |
Protein Variants |
Reference |
---|
2.4.1.37 | A268T |
naturally occuring mutant from individuals with weak B phenotype showing serologically weak B antigen on their red cells, overview |
695221 |
2.4.1.37 | A268T |
naturally occurring mutant from individuals with weak B phenotype showing serologically weak B antigen on their red cells, overview |
695221 |
2.4.1.37 | C209A |
site-directed mutagenesis, mutant structure determination |
690230 |
2.4.1.37 | C80S/C196S |
both the double and triple mutants show differing levels of disorder depending on their liganded state, with the level of disorder increasing when substrates are bound.The double mutant shows disorder over residues 177-180 in the unliganded and H-antigen bound forms, with disorder increasing to residues 176-185 for the UDP-, UDP + H-, and UDP-alpha-D-galactose + 3-deoxy-Gal inactive acceptor analog-bound structures. The double mutant has a reduction in Km for both donor and acceptor substrates. Mutation shows little effect over kcat |
720246 |
2.4.1.37 | C80S/C196S/C209S |
both the double and triple mutants show differing levels of disorder depending on their liganded state, with the level of disorder increasing when substrates are bound.The unliganded triple mutant shows a nearly complete internal loop, with residues 176-181 disordered for the H-antigen-bound structure and 177-179 disordered for the UDP-bound structure. The UDP + H- and UDPGal + 3-deoxy-Gal inactive acceptor analog-bound structures of the triple mutant show an internal loop completely disordered over residues 176-184. The triple Cys-to-Ser mutant has an acceptor Km elevated approximately 5times over wild type, while the donor Km has doubled. Mutation shows little effect over kcat |
720246 |
2.4.1.37 | D262N |
naturally occuring mutant from individuals with weak B phenotype showing serologically weak B antigen on their red cells, overview |
695221 |
2.4.1.37 | D262N |
naturally occurring mutant from individuals with weak B phenotype showing serologically weak B antigen on their red cells, overview |
695221 |
2.4.1.37 | D302A |
almost complete loss of activity |
756890 |
2.4.1.37 | D302A/D316A |
strong decrease in kcat value |
756890 |
2.4.1.37 | D302C |
kcat value is 9% that of wild-type GTB |
756890 |