Crystallization (Comment) | Organism |
---|---|
structures of wild-type and mutants D302A, D302C, D302L, R188K. Conserved active site residues Arg188 and Asp302 are critical for catalysis, and disruption of their hydrogen bond network through mutation can dramatically decrease enzymatic activity | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D302A | almost complete loss of activity | Homo sapiens |
D302A/D316A | strong decrease in kcat value | Homo sapiens |
D302C | kcat value is 9% that of wild-type GTB | Homo sapiens |
D302E | kcat value is 47% that of wild-type GTB | Homo sapiens |
D302E/D316E | 50% decrease in kcat value | Homo sapiens |
D302L | almost complete loss of activity | Homo sapiens |
R188K | almost complete loss of activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
UDP-alpha-D-galactose | wild-type, pH 7.0, 37°C | Homo sapiens | |
0.053 | - |
UDP-alpha-D-galactose | mutant D302C, pH 7.0, 37°C | Homo sapiens | |
0.088 | - |
alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 | wild-type, pH 7.0, 37°C | Homo sapiens | |
0.1 | - |
alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 | mutant D302E/D316E, pH 7.0, 37°C | Homo sapiens | |
0.101 | - |
UDP-alpha-D-galactose | mutant R188K, pH 7.0, 37°C | Homo sapiens | |
0.101 | - |
alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 | mutant R188K, pH 7.0, 37°C | Homo sapiens | |
0.112 | - |
UDP-alpha-D-galactose | mutant D302E/D316E, pH 7.0, 37°C | Homo sapiens | |
0.117 | - |
alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 | mutant D302A/D316A, pH 7.0, 37°C | Homo sapiens | |
0.17 | - |
UDP-alpha-D-galactose | mutant D302A/D316A, pH 7.0, 37°C | Homo sapiens | |
0.332 | - |
alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 | mutant D302C, pH 7.0, 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P16422 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-galactose + alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 | - |
Homo sapiens | UDP + alpha-D-Gal-(1->3)-[alpha-Fuc-(1->2)]-beta-D-Gal-(CH2)7CH3 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 | mutant R188K, pH 7.0, 37°C | Homo sapiens | |
0.002 | - |
alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 | mutant D302A/D316A, pH 7.0, 37°C | Homo sapiens | |
0.46 | - |
alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 | mutant D302C, pH 7.0, 37°C | Homo sapiens | |
2.4 | - |
alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 | mutant D302E/D316E, pH 7.0, 37°C | Homo sapiens | |
5.1 | - |
alpha-Fuc-(1->2)-beta-D-Gal-(CH2)7CH3 | wild-type, pH 7.0, 37°C | Homo sapiens |