EC Number   |
Protein Variants |
Reference |
|---|
   2.4.1.100 | D281Y |
the mutation restores the tight H-bond network and introduces typical S-type enzyme characteristics like producing similar amounts of 1-kestotriose compared to sucrose:sucrose 1-fructosyltransferases |
704883 |
| 2.4.1.100 | H308T |
site-directed mutagenesis |
736159 |
| 2.4.1.100 | M19F |
site-directed mutagenesis |
736159 |
| 2.4.1.100 | N340D |
the mutant shows only very low overall activity levels |
706297 |
| 2.4.1.100 | N340D/S415N |
the mutant shows similar properties to the W343R mutant, but the 1-nystose/neoketose ratio is decreased |
706297 |
| 2.4.1.100 | N340D/W343R |
the mutant shows 6G-FFT and 1-FFT activities that hold the middle between those of the N340D and W343R single mutants |
706297 |
| 2.4.1.100 | N340D/W343R/S415N |
the triple mutant gains the characteristic of using sucrose as a donor substrate, at the same time, this mutant can still use 1-kestose as a donor substrate, but in a much less efficient way than the wild type |
706297 |
| 2.4.1.100 | S415N |
the mutant shows slightly lower 6G-FFT/1-FFT activities, but the ratio between the main reaction products is not significantly affected |
706297 |
| 2.4.1.100 | W343R |
the mutant shows a wild type1-FFT activity but a largely reduced 6G-FFT activity |
706297 |
