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Literature summary for 2.4.1.100 extracted from

  • De Sadeleer, E.; Vergauwen, R.; Struyf, T.; Le Roy, K.; Van den Ende, W.
    1-FFT amino acids involved in high DP inulin accumulation in Viguiera discolor (2015), Front. Plant Sci., 6, 616.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.100

Cloned(Commentary)

Cloned (Comment) Organism
gene fft, sequence comparisons, recombinant expression of wild-type and mutant enzymes in Pichia pastoris Viguiera discolor
gene fft-1, sequence comparisons, recombinant expression in Pichia pastoris Helianthus tuberosus

Protein Variants

Protein Variants Comment Organism
H308T site-directed mutagenesis Viguiera discolor
M19F site-directed mutagenesis Viguiera discolor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Helianthus tuberosus inulins, one of the fructan types with beta(2-1)-linkages, are elongated by fructan:fructan1-fructosyltransferase (1-FFT) using a fructosyl unit from a donor inulin to elongate the acceptor inulin molecule ?
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 ?
additional information Viguiera discolor inulins, one of the fructan types with beta(2-1)-linkages, are elongated by fructan:fructan1-fructosyltransferase (1-FFT) using a fructosyl unit from a donor inulin to elongate the acceptori inulin molecule ?
-
 ?

Organism

Organism UniProt Comment Textmining
Helianthus tuberosus O81985 gene fft-1
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Viguiera discolor Q2WEC6 gene fft
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information inulins, one of the fructan types with beta(2-1)-linkages, are elongated by fructan:fructan1-fructosyltransferase (1-FFT) using a fructosyl unit from a donor inulin to elongate the acceptor inulin molecule Helianthus tuberosus ?
-
 ?
additional information inulins, one of the fructan types with beta(2-1)-linkages, are elongated by fructan:fructan1-fructosyltransferase (1-FFT) using a fructosyl unit from a donor inulin to elongate the acceptori inulin molecule Viguiera discolor ?
-
 ?
additional information 1-kestotriose is used as sole substrate, product analysis of wild-type and mutant enzyme reactions, overview Viguiera discolor ?
-
 ?
additional information 1-kestotriose is used as sole substrate, product analysis, overview Helianthus tuberosus ?
-
 ?

Synonyms

Synonyms Comment Organism
1-FFT
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 Viguiera discolor
1-FFT
-
 Helianthus tuberosus
fructan:fructan 1-fructosyltransferase
-
 Viguiera discolor
fructan:fructan 1-fructosyltransferase
-
 Helianthus tuberosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 recombinant enzyme, 5 min, inactivation Viguiera discolor
90
-
 recombinant enzyme, 5 min, inactivation Helianthus tuberosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 assay at Viguiera discolor
5
-
 assay at Helianthus tuberosus

General Information

General Information Comment Organism
evolution the enzyme is a member of the glycoside hydrolase family 32 Viguiera discolor
evolution the enzyme is a member of the glycoside hydrolase family 32 Helianthus tuberosus
metabolism inulin synthesis is the result of the combined action of sucrose:sucrose 1-fructosyltransferase, 1-SST, EC 2.4.1.99, and fructan:fructan 1-fructosyltransferase, 1-FFT, where 1-SST catalyzes the formation of 1-kestotriose from two sucrose molecules, while releasing glucose. Secondly 1-FFT transfers fructosyl units from one inulin molecule to another to elongate the inulin chain Helianthus tuberosus
additional information three-dimensional structure and homology structure modeling based on the resolved three-dimensional structure of 6-SST/6-SFT from Pachysandra terminalis, overview Helianthus tuberosus
additional information three-dimensional structure and homology structure modeling, based on the resolved three-dimensional structure of 6-SST/6-SFT from Pachysandra terminalis, overview Viguiera discolor