EC Number   |
Protein Variants |
Reference |
|---|
   2.1.1.6 | C69S |
mutation makes variant 108V and 108M more sensitive to oxidation |
659347 |
| 2.1.1.6 | C95S |
mutation increases the activity measured in absence of dithiothreitol in both the 108V and the 108M variant |
659347 |
| 2.1.1.6 | D162A |
loss of S-adenosylmethionine binding |
-, 757023 |
| 2.1.1.6 | E106A |
loss of S-adenosylmethionine binding |
-, 757023 |
| 2.1.1.6 | H163A |
loss of S-adenosylmethionine binding |
-, 757023 |
| 2.1.1.6 | I56A |
dissociation constant of S-adenosylmethionine is similar to wild-type. Residue I56 forms a pair of H-bonds with oxygen in the methionine portion |
757023 |
| 2.1.1.6 | K144A |
mutation of the proposed catalytic Lys residue, does not abolish COMT activity |
755786 |
| 2.1.1.6 | K144A/V173Y |
shift towards para-selectivity |
755786 |
| 2.1.1.6 | L16P |
missense mutation |
706484 |
| 2.1.1.6 | M91I/Y95C |
active site humanized enzyme |
718479 |
