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Literature summary for 2.1.1.6 extracted from

  • Law, B.J.; Bennett, M.R.; Thompson, M.L.; Levy, C.; Shepherd, S.A.; Leys, D.; Micklefield, J.
    Effects of active-site modification and quaternary structure on the regioselectivity of catechol-O-methyltransferase (2016), Angew. Chem. Int. Ed. Engl., 55, 2683-2687 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.6

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant Y200L, with bound S-adenosyl-L-methionine and 3,5-dinitrocatechol, to 1.6 A resolution Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
K144A mutation of the proposed catalytic Lys residue, does not abolish COMT activity Rattus norvegicus
K144A/V173Y shift towards para-selectivity Rattus norvegicus
W38D mutation of hydrophobic pocket, approximately +93% regioisomeric excess, and around 50% conversion of substrate 3,4-dihydroxybenzaldehyde Rattus norvegicus
W38K mutant favors para- over meta-methylation of dihydroxy substrates Rattus norvegicus
W38R mutant favors para- over meta-methylation of dihydroxy substrates Rattus norvegicus
Y200L mutant favors meta- over para-methylation of dihydroxy substrates, and exhibits the greatest meta-selectivity whilst retaining relative activity similar to that of the wild-type Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P22734
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + (3,4-dihydroxyphenyl)acetic acid
-
 Rattus norvegicus S-adenosyl-L-homocysteine + (4-hydroxy-3-methoxyphenyl)acetic acid + (3-hydroxy-4-methoxyphenyl)acetic acid
-
 ?
S-adenosyl-L-methionine + 3,4-dihydroxybenzaldehyde
-
 Rattus norvegicus S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxybenzaldehyde + 3-hydroxy-4-methoxybenzaldehyde
-
 ?
S-adenosyl-L-methionine + 3,4-dihydroxybenzoic acid
-
 Rattus norvegicus S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxybenzoic acid + 3-hydroxy-4-methoxybenzoic acid
-
 ?
S-adenosyl-L-methionine + 4-nitrobenzene-1,2-diol
-
 Rattus norvegicus S-adenosyl-L-homocysteine + 2-methoxy-4-nitrophenol + 2-methoxy-5-nitrophenol
-
 ?
S-adenosyl-L-methionine + a catechol
-
 Rattus norvegicus S-adenosyl-L-homocysteine + a guaiacol
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 wild-type is independent of pH Rattus norvegicus