EC Number |
Protein Variants |
Reference |
---|
2.1.1.230 | D36A |
mutation significantly decreases activity |
711257 |
2.1.1.230 | E220Q |
mutant has 10% of activity compared to wild-type enzyme |
711257 |
2.1.1.230 | E35A |
mutation significantly decreases activity |
711257 |
2.1.1.230 | E91A |
mutation significantly decreases activity |
711257 |
2.1.1.230 | F88A |
mutation significantly decreases activity |
711257 |
2.1.1.230 | H258A/E259A |
double mutaion decreases methyltransferase activity to 40% compared to wild-type activity |
711257 |
2.1.1.230 | R135A |
mutation significantly decreases activity |
711257 |
2.1.1.230 | R135A |
site-directed mutagenesis, the inactive R135A mutant homodimer binds to the RNA causing a shift in electrophoretic mobility, and the heterodimer composed of active and inactive subunits also binds the RNA efficiently. The inactive heterodimer complex binds the RNA more efficiently than the inactive mutant homodimer |
737073 |
2.1.1.230 | R162A |
site-directed mutagenesis, the mutant binds RNA with 100fold weaker affinity than wild-type Tsr |
736455 |
2.1.1.230 | R165A |
mutation significantly decreases activity |
711257 |