EC Number |
Protein Variants |
Reference |
---|
1.2.1.22 | F180T |
renders an enzyme with the ability to use NADP+. NADP+ activity is higher than that attained with NAD+. Exhibits a 16fold increase in the Vm/Km ratio with NAD+ as the coenzyme. Absence of Mg2+ inhibitory effect on F180T activity |
695015 |
1.2.1.22 | H449R |
site-directed mutagenesis |
742119 |
1.2.1.22 | L158Y |
site-directed mutagenesis |
742119 |
1.2.1.22 | more |
calculated Gibbs free energy of protein-substrate interaction of enzyme mutants with D-glyceraldehyde as substrate |
742119 |
1.2.1.22 | N286A |
site-directed mutagenesis |
742119 |
1.2.1.22 | N286C |
site-directed mutagenesis |
742119 |
1.2.1.22 | N286D |
site-directed mutagenesis |
742119 |
1.2.1.22 | N286E |
engineering of L-lactaldehyde dehydrogenase. Contrary to wild-type, mutant is able to oxidate glyceraldehyde |
740187 |
1.2.1.22 | N286E |
site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme and is able to oxidate glyceraldehyde and methylglyoxal |
742119 |
1.2.1.22 | N286F |
site-directed mutagenesis |
742119 |