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Literature summary for 1.2.1.22 extracted from

  • Wu, X.; Xu, L.; Yan, M.
    A new NAD(+)-dependent glyceraldehyde dehydrogenase obtained by rational design of l-lactaldehyde dehydrogenase from Escherichia coli (2016), Biosci. Biotechnol. Biochem., 80, 2306-2310.
    View publication on PubMed
Search for more literature for 1.2.1.22

Protein Variants

Protein Variants Comment Organism
N286E engineering of L-lactaldehyde dehydrogenase. Contrary to wild-type, mutant is able to oxidate glyceraldehyde Escherichia coli
N286H engineering of L-lactaldehyde dehydrogenase. Contrary to wild-type, mutant is able to oxidate glyceraldehyde Escherichia coli
N286T engineering of L-lactaldehyde dehydrogenase. Contrary to wild-type, mutant is able to oxidate glyceraldehyde. Compared to L-lactaldehyde, N286T has a one-third lower Km value to glyceraldehyde. Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.27
-
 (S)-lactaldehyde mutant N286H, pH 7.0, 37°C Escherichia coli
5.8
-
 (S)-lactaldehyde mutant N286H, pH 7.0, 37°C Escherichia coli
7.5
-
 (S)-lactaldehyde mutant N286H, pH 7.0, 37°C Escherichia coli
14.5
-
 (S)-lactaldehyde wild-type, pH 7.0, 37°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P25553
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.74
-
 wild-type, pH 7.0, 37°C Escherichia coli
1.18
-
 mutant N286H, pH 7.0, 37°C Escherichia coli
1.78
-
 mutant N286H, pH 7.0, 37°C Escherichia coli
2.18
-
 mutant N286H, pH 7.0, 37°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactaldehyde + NAD+ + H2O
-
 Escherichia coli (S)-lactate + NADH + 2 H+
-
 ?

Synonyms

Synonyms Comment Organism
AldA
-
 Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 1 h, 70% residual activity for mutant N286T Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
761.5
-
 (S)-lactaldehyde wild-type, pH 7.0, 37°C Escherichia coli
1026
-
 (S)-lactaldehyde mutant N286H, pH 7.0, 37°C Escherichia coli
1545
-
 (S)-lactaldehyde mutant N286H, pH 7.0, 37°C Escherichia coli
1895
-
 (S)-lactaldehyde mutant N286H, pH 7.0, 37°C Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
52.5
-
 (S)-lactaldehyde wild-type, pH 7.0, 37°C Escherichia coli
206.1
-
 (S)-lactaldehyde mutant N286H, pH 7.0, 37°C Escherichia coli
326.8
-
 (S)-lactaldehyde mutant N286H, pH 7.0, 37°C Escherichia coli
808.1
-
 (S)-lactaldehyde mutant N286H, pH 7.0, 37°C Escherichia coli