EC Number   |
Protein Variants |
Reference  |
|---|
   1.13.11.66 | more |
expression of the hydroquinone dioxygenase PnpC1C2 multi-component protein complex in Escherichia coli results in conversion of hydroquinone to 4-hydroxymuconic semialdehyde |
-, 719104 |
| 1.13.11.66 | E278A |
mutation in putative Fe(II)-binding site, complete loss of activity |
719693 |
| 1.13.11.66 | H162A |
mutation in putative Fe(II)-binding site, complete loss of activity |
719693 |
| 1.13.11.66 | H229A |
mutation in putative Fe(II)-binding site, complete loss of activity |
719693 |
| 1.13.11.66 | E276A |
less than 6% of wild-type activity |
720066 |
| 1.13.11.66 | H11A |
less than 6% of wild-type activity |
720066 |
| 1.13.11.66 | H159A |
67% of wild-type activity |
720066 |
| 1.13.11.66 | H227A |
less than 6% of wild-type activity |
720066 |
| 1.13.11.66 | Y266F |
about 6% of wild-type activity |
720066 |
| 1.13.11.66 | E248Q |
site-directed mutagenesis, the mutation results in complete loss of enzyme activity |
745331 |
