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Literature summary for 1.13.11.66 extracted from

  • Miyauchi, K.; Adachi, Y.; Nagata, Y.; Takagi, M.
    Cloning and sequencing of a novel meta-cleavage dioxygenase gene whose product is involved in degradation of gamma-hexachlorocyclohexane in Sphingomonas paucimobilis (1999), J. Bacteriol., 181, 6712-6719.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sphingomonas paucimobilis

Protein Variants

Protein Variants Comment Organism
E278A mutation in putative Fe(II)-binding site, complete loss of activity Sphingomonas paucimobilis
H162A mutation in putative Fe(II)-binding site, complete loss of activity Sphingomonas paucimobilis
H229A mutation in putative Fe(II)-binding site, complete loss of activity Sphingomonas paucimobilis

Metals/Ions

Metals/Ions Comment Organism Structure
Iron sequence contains a putative Fe(II)-binding site Sphingomonas paucimobilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 x * 36000, SDS-PAGE Sphingomonas paucimobilis

Organism

Organism UniProt Comment Textmining
Sphingomonas paucimobilis Q9WXE6
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.27
-
 crude extract, substrate hydroquinone, pH 7.0, 37°C Sphingomonas paucimobilis
0.88
-
 crude extract, substrate 2-chlorohydroquinone, pH 7.0, 37°C Sphingomonas paucimobilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chlorohydroquinone + O2
-
 Sphingomonas paucimobilis ? ring cleavage product is an acylchloride, which reacts with water to give maleylacetate ?
hydroquinone + O2
-
 Sphingomonas paucimobilis 4-hydroxymuconic acid semialdehyde
-
 ?
additional information enzyme cleaves aromatic rings with two hydroxyl groups ar para positions preferably. No substrate: catechol Sphingomonas paucimobilis ?
-
 ?

Subunits

Subunits Comment Organism
? x * 36000, SDS-PAGE Sphingomonas paucimobilis

Synonyms

Synonyms Comment Organism
LinE
-
 Sphingomonas paucimobilis